Investigation of nanoparticles using magnetic resonance imaging after intravitreal injection

Hemalatha B. Raju, Ying Hu, Kyle Padgett, Jose E. Rodriguez, Jeffrey L. Goldberg

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Magnetic nanoparticles may be used for focal delivery for cells, plasmids or drugs, and other applications. Here we asked whether magnetic nanoparticles could be detected in vivo at different time points after intravitreal injection by magnetic resonance imaging. Methods: Adult Sprague-Dawley rats received intravitreal injections of 50-nm or 4-μm magnetic particles into the left eye, with an equal volume of phosphate-buffered saline into the right eye (as controls). Animals were examined by magnetic resonance imaging at 1h, 1day and 5weeks after injection. Eyes, brain, liver, spleen and kidney were also imaged with high-resolution ex vivo magnetic resonance imaging scanning. Results: In vivo magnetic resonance imaging at the 1h and 1day time points more clearly detected magnetic particles in the 4μm group compared with the 50-nm group, although 50-nm magnetic nanoparticles were easily visualized with high-resolution magnetic resonance imaging ex vivo. Five weeks after intravitreal injection magnetic resonance imaging clearly detected 4-μm particles inside the eye, but by this time point the 50-nm magnetic nanoparticles could not be detected by either in vivo or ex vivo high-resolution magnetic resonance imaging. No magnetic particles were detected in any other organ. Conclusions: Magnetic resonance imaging could be used to track magnetic nanoparticles in the eye with the dosing selected for this study. Clearance varies by size, with 50-nm magnetic nanoparticles cleared more quickly than 4-μm particles. Thus, nanoparticles may provide advantages over micron-scale particles when considering risks associated with long-term persistence.

Original languageEnglish
Pages (from-to)100-107
Number of pages8
JournalClinical and Experimental Ophthalmology
Volume40
Issue number1
DOIs
StatePublished - Feb 1 2012
Externally publishedYes

Fingerprint

Intravitreal Injections
Nanoparticles
Magnetic Resonance Imaging
Sprague Dawley Rats
Plasmids
Spleen
Phosphates
Kidney
Injections
Liver
Brain

Keywords

  • Imaging systems
  • Magnet
  • Nanoparticles
  • Toxicity

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Investigation of nanoparticles using magnetic resonance imaging after intravitreal injection. / Raju, Hemalatha B.; Hu, Ying; Padgett, Kyle; Rodriguez, Jose E.; Goldberg, Jeffrey L.

In: Clinical and Experimental Ophthalmology, Vol. 40, No. 1, 01.02.2012, p. 100-107.

Research output: Contribution to journalArticle

Raju, Hemalatha B. ; Hu, Ying ; Padgett, Kyle ; Rodriguez, Jose E. ; Goldberg, Jeffrey L. / Investigation of nanoparticles using magnetic resonance imaging after intravitreal injection. In: Clinical and Experimental Ophthalmology. 2012 ; Vol. 40, No. 1. pp. 100-107.
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