Abstract
Background: The function of RNA from the non-coding (the so called " dark matter" ) regions of the genome has been a subject of considerable recent debate. Perhaps the most controversy is regarding the function of RNAs found in introns of annotated transcripts, where most of the reads that map outside of exons are usually found. However, it has been reported that the levels of RNA in introns are minor relative to those of the corresponding exons, and that changes in the levels of intronic RNAs correlate tightly with that of adjacent exons. This would suggest that RNAs produced from the vast expanse of intronic space are just pieces of pre-mRNAs or excised introns en route to degradation.Results: We present data that challenges the notion that intronic RNAs are mere by-standers in the cell. By performing a highly quantitative RNAseq analysis of transcriptome changes during an inflammation time course, we show that intronic RNAs have a number of features that would be expected from functional, standalone RNA species. We show that there are thousands of introns in the mouse genome that generate RNAs whose overall abundance, which changes throughout the inflammation timecourse, and other properties suggest that they function in yet unknown ways.Conclusions: So far, the focus of non-coding RNA discovery has shied away from intronic regions as those were believed to simply encode parts of pre-mRNAs. Results presented here suggest a very different situation - the sequences encoded in the introns appear to harbor a yet unexplored reservoir of novel, functional RNAs. As such, they should not be ignored in surveys of functional transcripts or other genomic studies.
| Original language | English |
|---|---|
| Article number | 504 |
| Journal | BMC Genomics |
| Volume | 13 |
| Issue number | 1 |
| DOIs | |
| State | Published - Sep 24 2012 |
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ASJC Scopus subject areas
- Biotechnology
- Genetics
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Intronic RNAs constitute the major fraction of the non-coding RNA in mammalian cells. / St Laurent, Georges; Shtokalo, Dmitry; Tackett, Michael R.; Yang, Zhaoqing; Eremina, Tatyana; Wahlestedt, Claes R; Urcuqui-Inchima, Silvio; Seilheimer, Bernd; McCaffrey, Timothy A.; Kapranov, Philipp.
In: BMC Genomics, Vol. 13, No. 1, 504, 24.09.2012.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Intronic RNAs constitute the major fraction of the non-coding RNA in mammalian cells
AU - St Laurent, Georges
AU - Shtokalo, Dmitry
AU - Tackett, Michael R.
AU - Yang, Zhaoqing
AU - Eremina, Tatyana
AU - Wahlestedt, Claes R
AU - Urcuqui-Inchima, Silvio
AU - Seilheimer, Bernd
AU - McCaffrey, Timothy A.
AU - Kapranov, Philipp
PY - 2012/9/24
Y1 - 2012/9/24
N2 - Background: The function of RNA from the non-coding (the so called " dark matter" ) regions of the genome has been a subject of considerable recent debate. Perhaps the most controversy is regarding the function of RNAs found in introns of annotated transcripts, where most of the reads that map outside of exons are usually found. However, it has been reported that the levels of RNA in introns are minor relative to those of the corresponding exons, and that changes in the levels of intronic RNAs correlate tightly with that of adjacent exons. This would suggest that RNAs produced from the vast expanse of intronic space are just pieces of pre-mRNAs or excised introns en route to degradation.Results: We present data that challenges the notion that intronic RNAs are mere by-standers in the cell. By performing a highly quantitative RNAseq analysis of transcriptome changes during an inflammation time course, we show that intronic RNAs have a number of features that would be expected from functional, standalone RNA species. We show that there are thousands of introns in the mouse genome that generate RNAs whose overall abundance, which changes throughout the inflammation timecourse, and other properties suggest that they function in yet unknown ways.Conclusions: So far, the focus of non-coding RNA discovery has shied away from intronic regions as those were believed to simply encode parts of pre-mRNAs. Results presented here suggest a very different situation - the sequences encoded in the introns appear to harbor a yet unexplored reservoir of novel, functional RNAs. As such, they should not be ignored in surveys of functional transcripts or other genomic studies.
AB - Background: The function of RNA from the non-coding (the so called " dark matter" ) regions of the genome has been a subject of considerable recent debate. Perhaps the most controversy is regarding the function of RNAs found in introns of annotated transcripts, where most of the reads that map outside of exons are usually found. However, it has been reported that the levels of RNA in introns are minor relative to those of the corresponding exons, and that changes in the levels of intronic RNAs correlate tightly with that of adjacent exons. This would suggest that RNAs produced from the vast expanse of intronic space are just pieces of pre-mRNAs or excised introns en route to degradation.Results: We present data that challenges the notion that intronic RNAs are mere by-standers in the cell. By performing a highly quantitative RNAseq analysis of transcriptome changes during an inflammation time course, we show that intronic RNAs have a number of features that would be expected from functional, standalone RNA species. We show that there are thousands of introns in the mouse genome that generate RNAs whose overall abundance, which changes throughout the inflammation timecourse, and other properties suggest that they function in yet unknown ways.Conclusions: So far, the focus of non-coding RNA discovery has shied away from intronic regions as those were believed to simply encode parts of pre-mRNAs. Results presented here suggest a very different situation - the sequences encoded in the introns appear to harbor a yet unexplored reservoir of novel, functional RNAs. As such, they should not be ignored in surveys of functional transcripts or other genomic studies.
UR - http://www.scopus.com/inward/record.url?scp=84866508252&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84866508252&partnerID=8YFLogxK
U2 - 10.1186/1471-2164-13-504
DO - 10.1186/1471-2164-13-504
M3 - Article
C2 - 23006825
AN - SCOPUS:84866508252
VL - 13
JO - BMC Genomics
JF - BMC Genomics
SN - 1471-2164
IS - 1
M1 - 504
ER -