TY - JOUR
T1 - Intravital endoscopic technology for real-time monitoring of inflammation caused in experimental periodontitis
AU - Movila, Alexandru
AU - Kajiya, Mikihito
AU - Wisitrasameewong, Wichaya
AU - Stashenko, Philip
AU - Vardar-Sengul, Saynur
AU - Hernandez, Maria
AU - Thomas Temple, H.
AU - Kawai, Toshihisa
N1 - Funding Information:
This study was supported by The Forsyth Pilot grant ( FPL37 ) and NIH grants AG-053615 (A.M.), DE-03420 , DE-18499 , DE-19917 , and DE-007327 (T.K.) and by Massachusetts Life Science Center Competitive Capital Program (P.S.).
PY - 2018/6
Y1 - 2018/6
N2 - We report a novel method for in situ imaging of microvascular permeability in inflamed gingival tissue, using state-of-the-art Cellvizio™ intravital endoscopic technology and a mouse model of ligature-induced periodontitis. The silk ligature was first placed at the upper left second molar. Seven days later, the ligature was removed, and the animals were intravenously injected with Evans blue. Evans blue dye, which selectively binds to blood albumin, was used to monitor the level of inflammation by monitoring vascular permeability in control non-diseased and ligature-induced experimental periodontitis tissue. More specifically, leakage of Evans blue-bound albumin from the micro-capillary to connective tissue indicates the state of inflammation occurring in the specific site. Evans blue leakage from blood vessels was imaged in situ by directly attaching the endoscope (mini Z tip) of the Cellvizio™ system to the gingival tissue without any surgical incision. Evans blue emission intensity was significantly elevated in gingiva of periodontitis lesions, but not control non-ligature placed gingiva, indicating that this technology can be used as a potential minimally invasive diagnostic tool to monitor the level of inflammation at the periodontal disease site.
AB - We report a novel method for in situ imaging of microvascular permeability in inflamed gingival tissue, using state-of-the-art Cellvizio™ intravital endoscopic technology and a mouse model of ligature-induced periodontitis. The silk ligature was first placed at the upper left second molar. Seven days later, the ligature was removed, and the animals were intravenously injected with Evans blue. Evans blue dye, which selectively binds to blood albumin, was used to monitor the level of inflammation by monitoring vascular permeability in control non-diseased and ligature-induced experimental periodontitis tissue. More specifically, leakage of Evans blue-bound albumin from the micro-capillary to connective tissue indicates the state of inflammation occurring in the specific site. Evans blue leakage from blood vessels was imaged in situ by directly attaching the endoscope (mini Z tip) of the Cellvizio™ system to the gingival tissue without any surgical incision. Evans blue emission intensity was significantly elevated in gingiva of periodontitis lesions, but not control non-ligature placed gingiva, indicating that this technology can be used as a potential minimally invasive diagnostic tool to monitor the level of inflammation at the periodontal disease site.
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U2 - 10.1016/j.jim.2018.03.006
DO - 10.1016/j.jim.2018.03.006
M3 - Article
AN - SCOPUS:85044251346
VL - 457
SP - 26
EP - 29
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
SN - 0022-1759
ER -