Intranasal, rectal and intraperitoneal immunization with protoxin Cry1Ac from Bacillus thuringiensis induces compartmentalized serum, intestinal, vaginal and pulmonary immune responses in Balb/c mice

Leticia Moreno-Fierros, Normand García, Rafael Gutiérrez, Rubén López-Revilla, Roberto I Vazquez-Padron

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Recently we discovered that the Cry1Ac protoxin of Bacillus thuringiensis administered to Balb/c mice intraperitoneally (i.p.) or intragastrically is a systemic and intestinal immunogen as potent as cholera toxin. To further characterize the mucosal immunogenicity of Cry1Ac we additionally tried, the intranasal (i.n.) and rectal routes and used enzyme- linked immunoassays to determine anti-Cry1Ac antibody responses in the serum as well as in vaginal and tracheobronchial washes and in the fluids of the large and the small intestine. Immunization by the i.p., i.n. and rectal routes induced IgM, IgG and IgA antibodies in all the mucosal surfaces analyzed, but the magnitude and predominant isotype of each response depended on the route used and the mucosal site analyzed. These data extend our findings on the striking mucosal immunogencity of Cry1Ac and provide additional evidence on the compartmentalization of the mucosal immune system. (C) 2000 Editions scientifiques et medicales Elsevier SAS.

Original languageEnglish
Pages (from-to)885-890
Number of pages6
JournalMicrobes and Infection
Volume2
Issue number8
DOIs
StatePublished - Jul 1 2000
Externally publishedYes

Fingerprint

Cholera Toxin
Large Intestine
Immunoenzyme Techniques
Immunoglobulin A
Small Intestine
Antibody Formation
Immunoglobulin M
Anti-Idiotypic Antibodies
Immune System
Immunization
Immunoglobulin G
Lung
Antibodies
Serum
Bacillus thuringiensis protoxin

Keywords

  • Cry1Ac protoxin
  • Mucosal immune compartmentalization
  • Mucosal immunity
  • Vaginal immunity

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Intranasal, rectal and intraperitoneal immunization with protoxin Cry1Ac from Bacillus thuringiensis induces compartmentalized serum, intestinal, vaginal and pulmonary immune responses in Balb/c mice. / Moreno-Fierros, Leticia; García, Normand; Gutiérrez, Rafael; López-Revilla, Rubén; Vazquez-Padron, Roberto I.

In: Microbes and Infection, Vol. 2, No. 8, 01.07.2000, p. 885-890.

Research output: Contribution to journalArticle

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