Abstract
Recently we discovered that the Cry1Ac protoxin of Bacillus thuringiensis administered to Balb/c mice intraperitoneally (i.p.) or intragastrically is a systemic and intestinal immunogen as potent as cholera toxin. To further characterize the mucosal immunogenicity of Cry1Ac we additionally tried, the intranasal (i.n.) and rectal routes and used enzyme- linked immunoassays to determine anti-Cry1Ac antibody responses in the serum as well as in vaginal and tracheobronchial washes and in the fluids of the large and the small intestine. Immunization by the i.p., i.n. and rectal routes induced IgM, IgG and IgA antibodies in all the mucosal surfaces analyzed, but the magnitude and predominant isotype of each response depended on the route used and the mucosal site analyzed. These data extend our findings on the striking mucosal immunogencity of Cry1Ac and provide additional evidence on the compartmentalization of the mucosal immune system. (C) 2000 Editions scientifiques et medicales Elsevier SAS.
| Original language | English |
|---|---|
| Pages (from-to) | 885-890 |
| Number of pages | 6 |
| Journal | Microbes and Infection |
| Volume | 2 |
| Issue number | 8 |
| DOIs | |
| State | Published - Jul 1 2000 |
| Externally published | Yes |
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Keywords
- Cry1Ac protoxin
- Mucosal immune compartmentalization
- Mucosal immunity
- Vaginal immunity
ASJC Scopus subject areas
- Microbiology
- Infectious Diseases
Cite this
Intranasal, rectal and intraperitoneal immunization with protoxin Cry1Ac from Bacillus thuringiensis induces compartmentalized serum, intestinal, vaginal and pulmonary immune responses in Balb/c mice. / Moreno-Fierros, Leticia; García, Normand; Gutiérrez, Rafael; López-Revilla, Rubén; Vazquez-Padron, Roberto I.
In: Microbes and Infection, Vol. 2, No. 8, 01.07.2000, p. 885-890.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Intranasal, rectal and intraperitoneal immunization with protoxin Cry1Ac from Bacillus thuringiensis induces compartmentalized serum, intestinal, vaginal and pulmonary immune responses in Balb/c mice
AU - Moreno-Fierros, Leticia
AU - García, Normand
AU - Gutiérrez, Rafael
AU - López-Revilla, Rubén
AU - Vazquez-Padron, Roberto I
PY - 2000/7/1
Y1 - 2000/7/1
N2 - Recently we discovered that the Cry1Ac protoxin of Bacillus thuringiensis administered to Balb/c mice intraperitoneally (i.p.) or intragastrically is a systemic and intestinal immunogen as potent as cholera toxin. To further characterize the mucosal immunogenicity of Cry1Ac we additionally tried, the intranasal (i.n.) and rectal routes and used enzyme- linked immunoassays to determine anti-Cry1Ac antibody responses in the serum as well as in vaginal and tracheobronchial washes and in the fluids of the large and the small intestine. Immunization by the i.p., i.n. and rectal routes induced IgM, IgG and IgA antibodies in all the mucosal surfaces analyzed, but the magnitude and predominant isotype of each response depended on the route used and the mucosal site analyzed. These data extend our findings on the striking mucosal immunogencity of Cry1Ac and provide additional evidence on the compartmentalization of the mucosal immune system. (C) 2000 Editions scientifiques et medicales Elsevier SAS.
AB - Recently we discovered that the Cry1Ac protoxin of Bacillus thuringiensis administered to Balb/c mice intraperitoneally (i.p.) or intragastrically is a systemic and intestinal immunogen as potent as cholera toxin. To further characterize the mucosal immunogenicity of Cry1Ac we additionally tried, the intranasal (i.n.) and rectal routes and used enzyme- linked immunoassays to determine anti-Cry1Ac antibody responses in the serum as well as in vaginal and tracheobronchial washes and in the fluids of the large and the small intestine. Immunization by the i.p., i.n. and rectal routes induced IgM, IgG and IgA antibodies in all the mucosal surfaces analyzed, but the magnitude and predominant isotype of each response depended on the route used and the mucosal site analyzed. These data extend our findings on the striking mucosal immunogencity of Cry1Ac and provide additional evidence on the compartmentalization of the mucosal immune system. (C) 2000 Editions scientifiques et medicales Elsevier SAS.
KW - Cry1Ac protoxin
KW - Mucosal immune compartmentalization
KW - Mucosal immunity
KW - Vaginal immunity
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UR - http://www.scopus.com/inward/citedby.url?scp=0033942940&partnerID=8YFLogxK
U2 - 10.1016/S1286-4579(00)00398-1
DO - 10.1016/S1286-4579(00)00398-1
M3 - Article
VL - 2
SP - 885
EP - 890
JO - Microbes and Infection
JF - Microbes and Infection
SN - 1286-4579
IS - 8
ER -