Intraluminal tracheal occlusion using a modified 8-mm Z-stent in a sheep model of left-sided congenital diaphragmatic hernia

Christian Sosa-Sosa, Carlos Bermúdez, Ramen H. Chmait, Eftichia Kontopoulos, Yvette Córdoba, Freddy Guevara-Zuloaga, Thora Steffensen, Rubén A. Quintero

Research output: Contribution to journalArticle

Abstract

Objective: To evaluate pulmonary growth and development after fetoscopic intraluminal tracheal occlusion (FITO) using a modified 8-mm Z-stent in an ovine model of congenital left-sided diaphragmatic hernia (CDH). Methods: Thirty-three time-dated ewes were studied: Group I: healthy controls; Group II: CDH controls (untreated); Group III: CDH treated with FITO. CDH was created in Groups II and III at 7080 days' gestation. FITO was performed at 100110 days. Left lung histological, morphometric, immunohistochemical and biochemical studies were conducted after delivery and euthanasia at 138 days. Results: Fifteen (45%) animals (Group I: 3; Group II: 5; Group III: 7) were available for analysis. The left lung parenchymal volume to fetal weight ratios were similar between Groups I and III (p = 0.24), and higher than Group II (p < 0.05III (79 versus 75%, p = 0.26), compared to 41% in Group II (p < 0.05). Pulmonary hypoplasia occurred in 1/7 (16%) in the FITO group, compared to 100% in Group II and 0% in Group I (p = .003). DNA and protein were significantly increased in Group III (p < 0.001). The concentration of type II pneumocytes was similar between healthy controls and the FITO group, and was paradoxically increased in untreated hernia fetuses. There was no histological evidence of tracheal injury. Conclusion: FITO with a modified 8-mm Z-stent is associated with lung growth and maturation similar to controls without obvious deleterious effects. A phase I clinical trial of FITO with the modified 8-mm Z-stent in severe CDH patients seems warranted.

Original languageEnglish (US)
Pages (from-to)2346-2353
Number of pages8
JournalJournal of Maternal-Fetal and Neonatal Medicine
Volume25
Issue number11
DOIs
StatePublished - Nov 1 2012

Keywords

  • CDH
  • Congenital diaphragmatic hernia
  • Fetal surgery
  • Fetoscopy
  • Tracheal occlusion

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Obstetrics and Gynecology

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  • Cite this

    Sosa-Sosa, C., Bermúdez, C., Chmait, R. H., Kontopoulos, E., Córdoba, Y., Guevara-Zuloaga, F., Steffensen, T., & Quintero, R. A. (2012). Intraluminal tracheal occlusion using a modified 8-mm Z-stent in a sheep model of left-sided congenital diaphragmatic hernia. Journal of Maternal-Fetal and Neonatal Medicine, 25(11), 2346-2353. https://doi.org/10.3109/14767058.2012.695825