Intracellular adriamycin levels and cytotoxicity in adriamycin-sensitive and adriamycin-resistant P388 mouse leukemia cells

R. Ganapathi, W. Reiter, A. Krishan

Research output: Contribution to journalArticle

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Abstract

Adriamycin (ADR) (NSC-123127) uptake and retention in ADR-sensitive P388 leukemia (P388/S) and ADR-resistant P388 leukemia (P388/R) cells were compared by fluorometry and laser flow cytometry (FCM) and were correlated with cytotoxic effects. Drug levels in P388/R cells treated in vitro with ADR (1-10 μg/ml) were twofold to fourfold lower than were levels in similarly treated P388/S cells. FCM analysis of P388/S and P388/R cells exposed in vitro to ADR showed qualitative and quantitative differences in ADR fluorescence profiles of drug-treated cells (1-5 μg/ml) but not of the isolated nuclei (0.5-10 μg/ml). Drug-induced perturbations in cell cycle traverse and chromosome aberrations were seen in P388/S but not in P388/R cells treated with 0.5-5 μg ADR/ml in vitro or 4-8 mg ADR/kg in vivo. The role of FCM in rapidly comparing and quantitating cellular ADR fluorescence profiles of ADR-sensitive and ADR-resistant tumors was demonstrated.

Original languageEnglish
Pages (from-to)1027-1032
Number of pages6
JournalJournal of the National Cancer Institute
Volume68
Issue number6
StatePublished - Jan 1 1982
Externally publishedYes

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Leukemia P388
Doxorubicin
Flow Cytometry
Fluorescence
Pharmaceutical Preparations
Fluorometry
Chromosome Aberrations
Cell Cycle
Lasers

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Intracellular adriamycin levels and cytotoxicity in adriamycin-sensitive and adriamycin-resistant P388 mouse leukemia cells. / Ganapathi, R.; Reiter, W.; Krishan, A.

In: Journal of the National Cancer Institute, Vol. 68, No. 6, 01.01.1982, p. 1027-1032.

Research output: Contribution to journalArticle

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