Intracellular adriamycin levels and cytotoxicity in adriamycin-sensitive and adriamycin-resistant p388 mouse leukemia cells

Ram Ganapathi, William Reiter, Awtar Krishan

Research output: Contribution to journalArticle

58 Scopus citations

Abstract

Adriamycin (ADR) (NSC-123127) uptake and retention in ADR-sensitive P388 leukemia (P388/S) and ADR-resistant P388 leukemia (P388/R) cells were compared by fluorometry and laser flow cytometry (FCM) and were correlated with cytotoxic effects. Drug levels in P388/R cells treated in vitro with ADR (1-10 μg/ml) were twofold to fourfold lower than were levels in similarly treated P388/S cells. FCM analysis of P388/S and P388/R cells exposed in vitro to ADR showed qualitative and quantitative differences in ADR fluorescence profiles of drug-treated cells (1-5 μg/ml) but not of the isolated nuclei (0.5-10 μg/ml). Drug-induced perturbations in cell cycle traverse and chromosome aberrations were seen in P388/S but not in P388/R cells treated with 0.5-5 μg ADR/ml in vitro or 4-8 mg ADR/kg in vivo. The role of FCM in rapidly comparing and quantitating cellular ADR fluorescence profiles of ADR-sensitive and ADR-resistant tumors was demonstrated.

Original languageEnglish (US)
Pages (from-to)1027-1032
Number of pages6
JournalJournal of the National Cancer Institute
Volume68
Issue number6
DOIs
StatePublished - Jun 1982

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Intracellular adriamycin levels and cytotoxicity in adriamycin-sensitive and adriamycin-resistant p388 mouse leukemia cells'. Together they form a unique fingerprint.

  • Cite this