Intestinal transport following transfer to increased salinity in an anadromous fish (Oncorhynchus mykiss)

Janet Genz, Andrew J. Esbaugh, Martin Grosell

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The ability to transition from freshwater to seawater environments is an intrinsic requirement of the life history of some fish species, including the anadromous rainbow trout (Oncorhynchus mykiss). The differences between hyper- and hypoosmoregulation are developed quickly (in hours to days), and at all scales, from gene expression to organ function. In this study, intestinal ion and water transport was examined in O. mykiss following acute transfer from freshwater (FW) to 70% seawater (SW). Plasma [Mg2+] increased at 24h post-transfer but recovered by 72h. In the intestinal fluids, total CO2 was found to increase with SW exposure/acclimation, while [Na+] decreased after 24h of SW exposure. Overall, in vitro experiments demonstrated the importance of base secretion to epithelial water uptake, and suggested that the primary physiological adjustments occurred 24-72h after acute SW transfer. The mRNA expression of ion transporters important for intestinal osmoregulation and maintenance of acid-base balance was also investigated. A Na+/H+ exchanger (NHE2) and anion exchanger (SLC26a6) were hypothesized to be involved in the transport of acid-base equivalents, Na+, and Cl-, but were not uniformly expressed across tissue samples, and expression, where present, did not change following salinity transfer. NHE1, however, was expressed in all examined tissues (gill, kidney, anterior intestine, and pyloric cecae), but exhibited no changes in expression following acute salinity transfer.

Original languageEnglish
Pages (from-to)150-158
Number of pages9
JournalComparative Biochemistry and Physiology - A Molecular and Integrative Physiology
Volume159
Issue number2
DOIs
StatePublished - Jun 1 2011

Fingerprint

Oncorhynchus mykiss
Salinity
Seawater
Fish
Fishes
Fresh Water
Head Kidney
Ions
Tissue
Osmoregulation
Sodium-Hydrogen Antiporter
Acid-Base Equilibrium
Water
Acclimatization
Ion Transport
Gene expression
Intestines
Anions
Maintenance
Plasmas

Keywords

  • Base secretion
  • NHE1
  • NHE2
  • Rainbow trout
  • SLC26a6

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Physiology

Cite this

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abstract = "The ability to transition from freshwater to seawater environments is an intrinsic requirement of the life history of some fish species, including the anadromous rainbow trout (Oncorhynchus mykiss). The differences between hyper- and hypoosmoregulation are developed quickly (in hours to days), and at all scales, from gene expression to organ function. In this study, intestinal ion and water transport was examined in O. mykiss following acute transfer from freshwater (FW) to 70{\%} seawater (SW). Plasma [Mg2+] increased at 24h post-transfer but recovered by 72h. In the intestinal fluids, total CO2 was found to increase with SW exposure/acclimation, while [Na+] decreased after 24h of SW exposure. Overall, in vitro experiments demonstrated the importance of base secretion to epithelial water uptake, and suggested that the primary physiological adjustments occurred 24-72h after acute SW transfer. The mRNA expression of ion transporters important for intestinal osmoregulation and maintenance of acid-base balance was also investigated. A Na+/H+ exchanger (NHE2) and anion exchanger (SLC26a6) were hypothesized to be involved in the transport of acid-base equivalents, Na+, and Cl-, but were not uniformly expressed across tissue samples, and expression, where present, did not change following salinity transfer. NHE1, however, was expressed in all examined tissues (gill, kidney, anterior intestine, and pyloric cecae), but exhibited no changes in expression following acute salinity transfer.",
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