Intestinal iron uptake in the European flounder (Platichthys flesus)

Nic R. Bury, Martin Grosell, C. M. Wood, C. Hogstrand, R. W. Wilson, J. C. Rankin, M. Busk, T. Lecklin, F. B. Jensen

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Iron is an essential element because it is a key constituent of the metalloproteins involved in cellular respiration and oxygen transport. There is no known regulated excretory mechanism for iron, and homeostasis is tightly controlled via its uptake from the diet. This study assessed in vivo intestinal iron uptake and in vitro iron absorption in a marine teleost, the European flounder Platichthys flesus. Ferric iron, in the form 59FeCl3, was reduced to Fe2+ by ascorbate, and the bioavailability of Fe3+ and Fe2+ were compared. In vivo Fe2+ uptake was significantly greater than Fe3+ uptake and was reduced by the iron chelator desferrioxamine. Fe2+ was also more bioavailable than Fe3+ in in vitro studies that assessed the temporal pattern and concentration-dependency of iron absorption. The posterior region, when compared with the anterior and mid regions of the intestine, was the preferential site for Fe2+ uptake in vivo. In vitro iron absorption was upregulated in the posterior intestine in response to prior haemoglobin depletion of the fish, and the transport showed a Q10 value of 1.94. Iron absorption in the other segments of the intestine did not correlate with haematocrit, and Q10 values were lower. Manipulation of the luminal pH had no effect on in vitro iron absorption. The present study demonstrates that a marine teleost absorbs Fe2+ preferentially in the posterior intestine. This occurs in spite of extremely high luminal bicarbonate concentrations recorded in vivo, which may be expected to reduce the bioavailability of divalent cations as a result of the precipitation as carbonates (e.g. FeCO3).

Original languageEnglish
Pages (from-to)3779-3787
Number of pages9
JournalJournal of Experimental Biology
Volume204
Issue number21
StatePublished - Dec 1 2001
Externally publishedYes

Fingerprint

Flounder
Platichthys flesus
flounder
Iron
iron
uptake mechanisms
iron absorption
Intestines
intestines
teleost
Biological Availability
bioavailability
Metalloproteins
Cell Respiration
metalloproteins
deferoxamine
Deferoxamine
Carbonates
Divalent Cations
homeostasis

Keywords

  • Bioavailability
  • European flounder
  • Intestine
  • Iron
  • Platichthys flesus

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Bury, N. R., Grosell, M., Wood, C. M., Hogstrand, C., Wilson, R. W., Rankin, J. C., ... Jensen, F. B. (2001). Intestinal iron uptake in the European flounder (Platichthys flesus). Journal of Experimental Biology, 204(21), 3779-3787.

Intestinal iron uptake in the European flounder (Platichthys flesus). / Bury, Nic R.; Grosell, Martin; Wood, C. M.; Hogstrand, C.; Wilson, R. W.; Rankin, J. C.; Busk, M.; Lecklin, T.; Jensen, F. B.

In: Journal of Experimental Biology, Vol. 204, No. 21, 01.12.2001, p. 3779-3787.

Research output: Contribution to journalArticle

Bury, NR, Grosell, M, Wood, CM, Hogstrand, C, Wilson, RW, Rankin, JC, Busk, M, Lecklin, T & Jensen, FB 2001, 'Intestinal iron uptake in the European flounder (Platichthys flesus)', Journal of Experimental Biology, vol. 204, no. 21, pp. 3779-3787.
Bury NR, Grosell M, Wood CM, Hogstrand C, Wilson RW, Rankin JC et al. Intestinal iron uptake in the European flounder (Platichthys flesus). Journal of Experimental Biology. 2001 Dec 1;204(21):3779-3787.
Bury, Nic R. ; Grosell, Martin ; Wood, C. M. ; Hogstrand, C. ; Wilson, R. W. ; Rankin, J. C. ; Busk, M. ; Lecklin, T. ; Jensen, F. B. / Intestinal iron uptake in the European flounder (Platichthys flesus). In: Journal of Experimental Biology. 2001 ; Vol. 204, No. 21. pp. 3779-3787.
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