Intestinal carbonic anhydrase, bicarbonate, and proton carriers play a role in the acclimation of rainbow trout to seawater

Martin Grosell, Katie M. Gilmour, Steven F. Perry

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Abrupt transfer of rainbow trout from freshwater to 65% seawater caused transient disturbances in extracellular fluid ionic composition, but homeostasis was reestablished 48 h posttransfer. Intestinal fluid chemistry revealed early onset of drinking and slightly delayed intestinal water absorption that coincided with initiation of NaCl absorption and HCO3- secretion. Suggestive of involvement in osmoregulation, relative mRNA levels for vacuolar H+-ATPase (V-ATPase), Na+-K+-ATPase, Na+/H+ exchanger 3 (NHE3), Na+-HCO 3- cotransporter 1, and two carbonic anhydrase (CA) isoforms [a general cytosolic isoform trout cytoplasmic CA (tCAc) and an extracellular isoform trout membrane-bound CA type IV (tCAIV)], were increased transiently in the intestine following exposure to 65% seawater. Both tCAc and tCAIV proteins were localized to apical regions of the intestinal epithelium and exhibited elevated enzymatic activity after acclimation to 65% seawater. The V-ATPase was localized to both basolateral and apical regions and exhibited a 10-fold increase in enzymatic activity in fish acclimated to 65% seawater, suggesting a role in marine osmoregulation. The intestinal epithelium of rainbow trout acclimated to 65% seawater appears to be capable of both basolateral and apical H+ extrusion, likely depending on osmoregulatory status and intestinal fluid chemistry.

Original languageEnglish
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume293
Issue number5
DOIs
StatePublished - Nov 1 2007

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Carbonic Anhydrases
Oncorhynchus mykiss
Acclimatization
Seawater
Bicarbonates
Protons
Trout
Vacuolar Proton-Translocating ATPases
Osmoregulation
Protein Isoforms
Intestinal Mucosa
Carbonic Anhydrase IV
Sodium-Hydrogen Antiporter
Extracellular Fluid
Intestinal Absorption
Fresh Water
Drinking
Intestines
Fishes
Homeostasis

Keywords

  • HCO secretion
  • Intestinal H transport
  • tCAc
  • tCAIV
  • Water absorption

ASJC Scopus subject areas

  • Physiology

Cite this

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abstract = "Abrupt transfer of rainbow trout from freshwater to 65{\%} seawater caused transient disturbances in extracellular fluid ionic composition, but homeostasis was reestablished 48 h posttransfer. Intestinal fluid chemistry revealed early onset of drinking and slightly delayed intestinal water absorption that coincided with initiation of NaCl absorption and HCO3- secretion. Suggestive of involvement in osmoregulation, relative mRNA levels for vacuolar H+-ATPase (V-ATPase), Na+-K+-ATPase, Na+/H+ exchanger 3 (NHE3), Na+-HCO 3- cotransporter 1, and two carbonic anhydrase (CA) isoforms [a general cytosolic isoform trout cytoplasmic CA (tCAc) and an extracellular isoform trout membrane-bound CA type IV (tCAIV)], were increased transiently in the intestine following exposure to 65{\%} seawater. Both tCAc and tCAIV proteins were localized to apical regions of the intestinal epithelium and exhibited elevated enzymatic activity after acclimation to 65{\%} seawater. The V-ATPase was localized to both basolateral and apical regions and exhibited a 10-fold increase in enzymatic activity in fish acclimated to 65{\%} seawater, suggesting a role in marine osmoregulation. The intestinal epithelium of rainbow trout acclimated to 65{\%} seawater appears to be capable of both basolateral and apical H+ extrusion, likely depending on osmoregulatory status and intestinal fluid chemistry.",
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AU - Gilmour, Katie M.

AU - Perry, Steven F.

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N2 - Abrupt transfer of rainbow trout from freshwater to 65% seawater caused transient disturbances in extracellular fluid ionic composition, but homeostasis was reestablished 48 h posttransfer. Intestinal fluid chemistry revealed early onset of drinking and slightly delayed intestinal water absorption that coincided with initiation of NaCl absorption and HCO3- secretion. Suggestive of involvement in osmoregulation, relative mRNA levels for vacuolar H+-ATPase (V-ATPase), Na+-K+-ATPase, Na+/H+ exchanger 3 (NHE3), Na+-HCO 3- cotransporter 1, and two carbonic anhydrase (CA) isoforms [a general cytosolic isoform trout cytoplasmic CA (tCAc) and an extracellular isoform trout membrane-bound CA type IV (tCAIV)], were increased transiently in the intestine following exposure to 65% seawater. Both tCAc and tCAIV proteins were localized to apical regions of the intestinal epithelium and exhibited elevated enzymatic activity after acclimation to 65% seawater. The V-ATPase was localized to both basolateral and apical regions and exhibited a 10-fold increase in enzymatic activity in fish acclimated to 65% seawater, suggesting a role in marine osmoregulation. The intestinal epithelium of rainbow trout acclimated to 65% seawater appears to be capable of both basolateral and apical H+ extrusion, likely depending on osmoregulatory status and intestinal fluid chemistry.

AB - Abrupt transfer of rainbow trout from freshwater to 65% seawater caused transient disturbances in extracellular fluid ionic composition, but homeostasis was reestablished 48 h posttransfer. Intestinal fluid chemistry revealed early onset of drinking and slightly delayed intestinal water absorption that coincided with initiation of NaCl absorption and HCO3- secretion. Suggestive of involvement in osmoregulation, relative mRNA levels for vacuolar H+-ATPase (V-ATPase), Na+-K+-ATPase, Na+/H+ exchanger 3 (NHE3), Na+-HCO 3- cotransporter 1, and two carbonic anhydrase (CA) isoforms [a general cytosolic isoform trout cytoplasmic CA (tCAc) and an extracellular isoform trout membrane-bound CA type IV (tCAIV)], were increased transiently in the intestine following exposure to 65% seawater. Both tCAc and tCAIV proteins were localized to apical regions of the intestinal epithelium and exhibited elevated enzymatic activity after acclimation to 65% seawater. The V-ATPase was localized to both basolateral and apical regions and exhibited a 10-fold increase in enzymatic activity in fish acclimated to 65% seawater, suggesting a role in marine osmoregulation. The intestinal epithelium of rainbow trout acclimated to 65% seawater appears to be capable of both basolateral and apical H+ extrusion, likely depending on osmoregulatory status and intestinal fluid chemistry.

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