Abstract
A total of 202 methicillin-resistant Staphylococcus aureus (MRSA) single-patient isolates recovered between January and June 1998 in two hospitals in Miami, Florida, were characterized by a combination of several molecular typing techniques: multilocus sequence typing, spaA typing, pulsed-field gel electrophoresis, and determination of the structure of the SCCmec element. The overwhelming majority of the isolates-187 of 202, or 93%-belonged to one of three internationally spread epidemic clones which were identified on the basis of their multilocus sequence type (ST) as E-MRSA-16 (ST36), the New York clone V (ST8), and the New York/Japan clone (ST5; SCCmec II) and its single- and double-locus variants. The rest of the isolates (15 of 202, or 7%) were more genetically diverse and were each recovered from a few patients only. Of the 23 MRSA strains isolated from confirmed human immunodeficiency virus-positive patients, as many as 17 (or 70%) belonged to a single ST8 clone carrying SCCmec type IV. The data provide further evidence for the conclusion of earlier studies that most MRSA disease in hospitals is caused by relatively few pandemic clones.
| Original language | English |
|---|---|
| Pages (from-to) | 542-547 |
| Number of pages | 6 |
| Journal | Journal of Clinical Microbiology |
| Volume | 42 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1 2004 |
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ASJC Scopus subject areas
- Microbiology (medical)
- Microbiology
Cite this
International Clones of Methicillin-Resistant Staphylococcus aureus in Two Hospitals in Miami, Florida. / Chung, Marilyn; Dickinson, Gordon; De Lencastre, Herminia; Tomasz, Alexander.
In: Journal of Clinical Microbiology, Vol. 42, No. 2, 01.02.2004, p. 542-547.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - International Clones of Methicillin-Resistant Staphylococcus aureus in Two Hospitals in Miami, Florida
AU - Chung, Marilyn
AU - Dickinson, Gordon
AU - De Lencastre, Herminia
AU - Tomasz, Alexander
PY - 2004/2/1
Y1 - 2004/2/1
N2 - A total of 202 methicillin-resistant Staphylococcus aureus (MRSA) single-patient isolates recovered between January and June 1998 in two hospitals in Miami, Florida, were characterized by a combination of several molecular typing techniques: multilocus sequence typing, spaA typing, pulsed-field gel electrophoresis, and determination of the structure of the SCCmec element. The overwhelming majority of the isolates-187 of 202, or 93%-belonged to one of three internationally spread epidemic clones which were identified on the basis of their multilocus sequence type (ST) as E-MRSA-16 (ST36), the New York clone V (ST8), and the New York/Japan clone (ST5; SCCmec II) and its single- and double-locus variants. The rest of the isolates (15 of 202, or 7%) were more genetically diverse and were each recovered from a few patients only. Of the 23 MRSA strains isolated from confirmed human immunodeficiency virus-positive patients, as many as 17 (or 70%) belonged to a single ST8 clone carrying SCCmec type IV. The data provide further evidence for the conclusion of earlier studies that most MRSA disease in hospitals is caused by relatively few pandemic clones.
AB - A total of 202 methicillin-resistant Staphylococcus aureus (MRSA) single-patient isolates recovered between January and June 1998 in two hospitals in Miami, Florida, were characterized by a combination of several molecular typing techniques: multilocus sequence typing, spaA typing, pulsed-field gel electrophoresis, and determination of the structure of the SCCmec element. The overwhelming majority of the isolates-187 of 202, or 93%-belonged to one of three internationally spread epidemic clones which were identified on the basis of their multilocus sequence type (ST) as E-MRSA-16 (ST36), the New York clone V (ST8), and the New York/Japan clone (ST5; SCCmec II) and its single- and double-locus variants. The rest of the isolates (15 of 202, or 7%) were more genetically diverse and were each recovered from a few patients only. Of the 23 MRSA strains isolated from confirmed human immunodeficiency virus-positive patients, as many as 17 (or 70%) belonged to a single ST8 clone carrying SCCmec type IV. The data provide further evidence for the conclusion of earlier studies that most MRSA disease in hospitals is caused by relatively few pandemic clones.
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UR - http://www.scopus.com/inward/citedby.url?scp=1242336867&partnerID=8YFLogxK
U2 - 10.1128/JCM.42.2.542-547.2004
DO - 10.1128/JCM.42.2.542-547.2004
M3 - Article
C2 - 14766814
AN - SCOPUS:1242336867
VL - 42
SP - 542
EP - 547
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
SN - 0095-1137
IS - 2
ER -