Purpose. We previously reported internal contact sclerostomy with an Er:YAG laser delivered through a fiber probe in enucleated porcine eyes. Our purpose was to evaluate the threshold and total energy required to create a full-thickness sclerostomy in eye-bank eyes. Method. We created sclerostomy on freshly enucleated eye bank eyes with a laboratory Er:YAG laser. The pulse duration was 200 μs and the pulse repetition rate was 2 Hz. The laser energy was delivered through a fluoride glass fiber terminated with a contact endoprobe. The probe consisted of a silica fiber protected with a nickel coating and the tip of the probe was tapered. Sclerostomies were created at 0.5, 1, 2, 4, and 8 mJ per pulse. We used a prototype GRIN endoscope (Volpi, Switzerland) with an intraocular diameter of 0.89 mm to accurately position the probe in the anterior chamber angle. The thermal damage was quantified by histology. Results. Full-thickness sclerostomies could be created at each energy level at approximately the same site in the angle. No significant difference in the total energy required for the sclerostomy was found between the groups with an energy per pulse of 1, 2, 4, and 8 mJ. With an energy per pulse of 0.5 mJ, the total energy required was significantly higher than at the other energy levels. The thermal damaged zone was approximately 15 μm in all groups. Conclusion. Full-thickness sclerostomies with little thermal damage could be made by internal contact sclerostomy with an Er:YAG laser. The intraocular endoscope provided an accurate control of the location of the sclerostomies.
|Original language||English (US)|
|Journal||Investigative Ophthalmology and Visual Science|
|State||Published - Feb 15 1996|
ASJC Scopus subject areas
- Sensory Systems
- Cellular and Molecular Neuroscience