H1V+ patients. We have previously reported that peripheral blood mononuclear cells(PBMC) from HIV-infected patients underwent marked spontaneous apoptosis in vitro. In this study, we examined the effect of IL-2 upon culture-induced apoptosis in PBMC from HIV infected patients by flow cy tome try. Here, we report that addition of IL-2 resulted in the reduction of spontaneous apoptosis in patient cells in both CD3+ and CD19lymphocytes. Interestingly, we observed that Bcl-2 expression in patient PBMC decreased rapidly upon in vitro culture while that in PBMC of healthy volunteers was relatively unaffected. The most significant decrease in Bcl-2 expression was noted in the apoptotic cell population. The apoptosis-inhibitory effect of IL-2 was found to be associated with the blocking of this cultureinduced down-modulation of Bcl-2 in patient PBMC. These data provide an experimental rationale for IL-2 therapy in slowing disease progression probably via its ability to block accelerated apoptosis in HIV infection.
|Original language||English (US)|
|State||Published - Dec 1 1996|
ASJC Scopus subject areas
- Molecular Biology