Interleukin-9 upregulates mucus expression in the airways

Jamila Louahed, Masao Toda, Jin Jen, Qutayba Hamid, Jean Cristophe Renauld, Roy C Levitt, Nicholas C. Nicolaides

Research output: Contribution to journalArticle

217 Citations (Scopus)

Abstract

Interleukin (IL)-9 has recently been shown to play an important role in allergic disease because its expression is strongly associated with the degree of airway responsiveness and the asthmatic-like phenotype. IL-9 is a pleiotropic cytokine that is active on many cell types involved in the allergic immune response. Mucus hypersecretion is a clinical feature of chronic airway diseases; however, the mechanisms underlying the induction of mucin are poorly understood. In this report, we show that IL-9 regulates the expression of a subset of mucin genes in lung cells both in vivo and in vitro. In vivo, the constitutive expression of IL-9 in transgenic mice results in elevated MUC2 and MUC5AC gene expression in airway epithelial cells and periodic acid-Schiff-positive staining (reflecting mucous glycogenates). Similar results were observed in C57BL/6J mice after IL-9 intratracheal instillation. In contrast, instillation of the T helper 1-associated cytokine interferon γ failed to induce mucin production. In vitro, our studies showed that IL-9 also induces expression of MUC2 and MUC5AC in human primary lung cultures and in the human muccoepidermoid NCI-H292 cell line, indicating a direct effect of IL-9 on inducing mucin expression in these cells. Altogether, these results suggest that upregulation of mucin by IL-9 might contribute to the pathogenesis of human inflammatory airway disorders, such as asthma. These data extend the role of the biologic processes that IL-9 has on regulating the many clinical features of asthma and further supports the IL-9 pathway as a key mediator of the asthmatic response.

Original languageEnglish
Pages (from-to)649-656
Number of pages8
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume22
Issue number6
StatePublished - Jul 6 2000
Externally publishedYes

Fingerprint

Interleukin-9
Mucus
Up-Regulation
Mucins
Asthma
Cytokines
Lung
Periodic Acid
Inbred C57BL Mouse
Cell culture
Gene expression
Interferons
Transgenic Mice
Chronic Disease
Genes
Epithelial Cells
Cells

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology

Cite this

Louahed, J., Toda, M., Jen, J., Hamid, Q., Renauld, J. C., Levitt, R. C., & Nicolaides, N. C. (2000). Interleukin-9 upregulates mucus expression in the airways. American Journal of Respiratory Cell and Molecular Biology, 22(6), 649-656.

Interleukin-9 upregulates mucus expression in the airways. / Louahed, Jamila; Toda, Masao; Jen, Jin; Hamid, Qutayba; Renauld, Jean Cristophe; Levitt, Roy C; Nicolaides, Nicholas C.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 22, No. 6, 06.07.2000, p. 649-656.

Research output: Contribution to journalArticle

Louahed, J, Toda, M, Jen, J, Hamid, Q, Renauld, JC, Levitt, RC & Nicolaides, NC 2000, 'Interleukin-9 upregulates mucus expression in the airways', American Journal of Respiratory Cell and Molecular Biology, vol. 22, no. 6, pp. 649-656.
Louahed, Jamila ; Toda, Masao ; Jen, Jin ; Hamid, Qutayba ; Renauld, Jean Cristophe ; Levitt, Roy C ; Nicolaides, Nicholas C. / Interleukin-9 upregulates mucus expression in the airways. In: American Journal of Respiratory Cell and Molecular Biology. 2000 ; Vol. 22, No. 6. pp. 649-656.
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