Interleukin-9 enhances interleukin-5 receptor expression, differentiation, and survival of human eosinophils

A. S. Gounni, B. Gregory, E. Nutku, F. Aris, K. Latifa, E. Minshall, J. North, J. Tavernier, R. Levit, N. Nicolaides, D. Robinson, Q. Hamid

Research output: Contribution to journalArticlepeer-review

108 Scopus citations

Abstract

Interleukin-9 (IL-9) has been implicated in the pathogenesis of allergic disorders. To examine the interaction between IL-9 and eosinophils, we evaluated mature peripheral blood eosinophils for their expression of the specific α-subunit of the IL-9 receptor (IL-9R-α). The expression of IL-9R-α by human eosinophils was detected at the messenger RNA (mRNA) and protein levels by reverse transcriptase-polymerase chain reaction (RT-PCR), flow cytometry, and immunocytochemical analysis, respectively. Functional analyses demonstrated that recombinant human (rh)IL-9 inhibited in vitro peripheral blood human eosinophil apoptosis in a concentration-dependent manner. We then examined the role of IL-9 in eosinophil differentiation using the human cord blood CD34+ cells and human promyelocytic leukemia cells (HL-60). The addition of IL-9 to CD34+ cells cultured in IL-3 and IL-5 enhanced eosinophil development, and IL-9 alone induced the expression of IL-5R-α. IL-9 also up-regulated the IL-5R-α chain cell surface expression during terminal eosinophil differentiation of the HL-60 cell line. Our findings suggest that IL-9 may potentiate in vivo eosinophil function by increasing their survival and IL-5-mediated differentiation and maturation. Taken together, these results suggest a mechanism by which IL-9 potentiates airway and tissue eosinophilia. (C) 2000 by The American Society of Hematology.

Original languageEnglish (US)
Pages (from-to)2163-2171
Number of pages9
JournalBlood
Volume96
Issue number6
DOIs
StatePublished - Sep 15 2000
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Interleukin-9 enhances interleukin-5 receptor expression, differentiation, and survival of human eosinophils'. Together they form a unique fingerprint.

Cite this