Interleukin-6 cdna transfected lewis lung carcinoma cells show unaltered net tumour growth rate but cause weight loss and shorten survival in syngenic mice

Y. Ohe, E. R. Podack, K. J. Olsen, Y. Miyahara, K. Miura, H. Saito, Y. Koishihara, Y. Ohsugi, T. Ohira, K. Nishio, N. Saijo

Research output: Contribution to journalArticlepeer-review

75 Scopus citations

Abstract

HuIL-6 cDNA, cloned into a neomycin resistant conferring expression vector, BMGNeo, was transfected into Lewis Lung Carcinoma (LLC) cells. LLC cells (5 × 106 ml-1) transfected with IL-6 cDNA (LLC-IL6) secreted IL-6 into the culture supernatant at a concentration of 9.9 ng ml-1 within 48 h. When 1,000,000 of untransfected LLC, BMGNeo vector transfected LLC (LLC-Neo) or LLC-IL6 cells were transplanted into C57BL/6 mice subcutaneously, the mean ± s.d. of survival times of these mice were 33.3 ± 9.7, 34.3 ± 7.1 and 17.0 ± 3.1 days, respectively. The survival time of LLC-IL6 cells transplanted mice was significantly shorter than that of LLC (P<0.01) or LLC-Neo (P<0.01) cells transplanted mice without a measurable difference of tumour size. Plasma concentration of IL-6 steadily increased in LLC-IL6 transplanted mice. Body weight and serum albumin were significantly lower in LLC-IL6 transplanted mice than in LLC transplanted mice. Mouse IL-1α and mouse TNF-α were not detected in the plasma of LLC-IL6 transplanted mice. These data suggested that secretion of IL-6 from LLC cells was unable to alter net tumour growth rate but rather caused a state similar to cachexia without detectable increase of IL-1α and TNF-α in the plasma. This state may be responsible for the shortened survival of LLC-116 tumour-bearing mice.

Original languageEnglish (US)
Pages (from-to)939-944
Number of pages6
JournalBritish Journal of Cancer
Volume67
Issue number5
DOIs
StatePublished - May 1993

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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