Abstract
Infection with human immunodeficiency virus (HIV)-1 induces a progressive deterioration of the immune system that ultimately leads to aquired immune deficiency syndrome (AIDS). Murine models indicate that the common γ-chain (γc)-sharing cytokine interleukin (IL)-21 and its receptor (IL-21R) play a crucial role in maintaining polyfunctional T cell responses during chronic viral infections. Therefore, we analyzed the ability of this cytokine to modulate the properties of human CD8 T cells in comparison with other γc-sharing cytokines (IL-2, IL-7, and IL-15). CD8 T cells from healthy volunteers were stimulated in vitro via T cell receptor signals to mimic the heightened status of immune activation of HIV-infected patients. The administration of IL-21 upregulated cytotoxic effector function and the expression of the costimulatory molecule CD28. Notably, this outcome was not accompanied by increased cellular proliferation or activation. Moreover, IL-21 promoted antiviral activity while not inducing HIV-1 replication in vitro. Thus, IL-21 may be a favorable molecule for immunotherapy and a suitable vaccine adjuvant in HIV-infected individuals.
| Original language | English |
|---|---|
| Pages (from-to) | 115-123 |
| Number of pages | 9 |
| Journal | Human Immunology |
| Volume | 72 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1 2011 |
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Keywords
- CD8 T lymphocytes
- Cytotoxicity
- HIV
- IL-21
- Immunotherapy
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
Cite this
Interleukin-21 and cellular activation concurrently induce potent cytotoxic function and promote antiviral activity in human CD8 T cells. / Parmigiani, Anita; Pallin, Maria F.; Schmidtmayerova, Helena; Lichtenheld, Mathias G; Pahwa, Savita G.
In: Human Immunology, Vol. 72, No. 2, 01.02.2011, p. 115-123.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Interleukin-21 and cellular activation concurrently induce potent cytotoxic function and promote antiviral activity in human CD8 T cells
AU - Parmigiani, Anita
AU - Pallin, Maria F.
AU - Schmidtmayerova, Helena
AU - Lichtenheld, Mathias G
AU - Pahwa, Savita G
PY - 2011/2/1
Y1 - 2011/2/1
N2 - Infection with human immunodeficiency virus (HIV)-1 induces a progressive deterioration of the immune system that ultimately leads to aquired immune deficiency syndrome (AIDS). Murine models indicate that the common γ-chain (γc)-sharing cytokine interleukin (IL)-21 and its receptor (IL-21R) play a crucial role in maintaining polyfunctional T cell responses during chronic viral infections. Therefore, we analyzed the ability of this cytokine to modulate the properties of human CD8 T cells in comparison with other γc-sharing cytokines (IL-2, IL-7, and IL-15). CD8 T cells from healthy volunteers were stimulated in vitro via T cell receptor signals to mimic the heightened status of immune activation of HIV-infected patients. The administration of IL-21 upregulated cytotoxic effector function and the expression of the costimulatory molecule CD28. Notably, this outcome was not accompanied by increased cellular proliferation or activation. Moreover, IL-21 promoted antiviral activity while not inducing HIV-1 replication in vitro. Thus, IL-21 may be a favorable molecule for immunotherapy and a suitable vaccine adjuvant in HIV-infected individuals.
AB - Infection with human immunodeficiency virus (HIV)-1 induces a progressive deterioration of the immune system that ultimately leads to aquired immune deficiency syndrome (AIDS). Murine models indicate that the common γ-chain (γc)-sharing cytokine interleukin (IL)-21 and its receptor (IL-21R) play a crucial role in maintaining polyfunctional T cell responses during chronic viral infections. Therefore, we analyzed the ability of this cytokine to modulate the properties of human CD8 T cells in comparison with other γc-sharing cytokines (IL-2, IL-7, and IL-15). CD8 T cells from healthy volunteers were stimulated in vitro via T cell receptor signals to mimic the heightened status of immune activation of HIV-infected patients. The administration of IL-21 upregulated cytotoxic effector function and the expression of the costimulatory molecule CD28. Notably, this outcome was not accompanied by increased cellular proliferation or activation. Moreover, IL-21 promoted antiviral activity while not inducing HIV-1 replication in vitro. Thus, IL-21 may be a favorable molecule for immunotherapy and a suitable vaccine adjuvant in HIV-infected individuals.
KW - CD8 T lymphocytes
KW - Cytotoxicity
KW - HIV
KW - IL-21
KW - Immunotherapy
UR - http://www.scopus.com/inward/record.url?scp=78651260395&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78651260395&partnerID=8YFLogxK
U2 - 10.1016/j.humimm.2010.10.015
DO - 10.1016/j.humimm.2010.10.015
M3 - Article
C2 - 20977918
AN - SCOPUS:78651260395
VL - 72
SP - 115
EP - 123
JO - Human Immunology
JF - Human Immunology
SN - 0198-8859
IS - 2
ER -