Interleukin 2 receptors on cultured murine epidermal Langerhans cells

G. Steiner, E. Tschachler, M. Tani, Thomas Malek, E. M. Shevach, W. Holter, W. Knapp, K. Wolff, G. Stingl

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Rat monoclonal antibodies 3C7 and 7D4 detect two distinct functional regions of the murine interleukin 2 (IL 2) receptor. When studying the emergence kinetics of IL 2 receptors in mixed epidermal cell (EC)-lymphocyte cultures by using 3C7 and 7D4 in an indirect immunofluorescence assay, we regularly encountered a distinctive membrane fluorescence not only on lymphocytes, but also on a subpopulation of cells exhibiting a dendritic morphology. Reasoning that these 3C7/7D4-reactive dendritic cells might represent a subpopulation of epidermal dendritic cells, we studied mouse EC for the presence of 3C7/7D4-reactive cells. Although 3C7/7D4 reactivity was never detected on freshly isolated EC or on epidermal sheets, a small number of 3C7/7D4+ cells was encountered after 24 to 48 hr of culture. These cells exhibited a dendritic shape, expressed Ia antigens, lacked Thy-1 antigens, and displayed the ultrastructural features of Langerhans cells (LC) with the notable exception of Birbeck granules. Although after 24 hr, only 20% of Ia+ EC were 3C7/7D4+, the vast majority of LC displayed 3C7/D74 binding sites after 4 to 5 days of culture. Preincubation of cultured LC-enriched EC with recombinant human IL 2 prevented subsequent 3C7-but not 7D4-binding to these cells. Western blot analysis of 7D4-reactive material of detergent extracts from LC-enriched EC revealed three bands in the same m.w. range as reported for CTLL cells. These results demonstrate that cultured LC express IL 2 receptors and may bear important implications for a better understanding of growth regulation, differentiation, and immunologic functions of LC.

Original languageEnglish
Pages (from-to)155-159
Number of pages5
JournalJournal of Immunology
Volume137
Issue number1
StatePublished - Sep 10 1986
Externally publishedYes

Fingerprint

Langerhans Cells
Interleukin-2 Receptors
Cultured Cells
Thy-1 Antigens
Lymphocytes
Histocompatibility Antigens Class II
Indirect Fluorescent Antibody Technique
Detergents
Dendritic Cells
Interleukin-2
Cell Culture Techniques
Fluorescence
Western Blotting
Binding Sites
Monoclonal Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Steiner, G., Tschachler, E., Tani, M., Malek, T., Shevach, E. M., Holter, W., ... Stingl, G. (1986). Interleukin 2 receptors on cultured murine epidermal Langerhans cells. Journal of Immunology, 137(1), 155-159.

Interleukin 2 receptors on cultured murine epidermal Langerhans cells. / Steiner, G.; Tschachler, E.; Tani, M.; Malek, Thomas; Shevach, E. M.; Holter, W.; Knapp, W.; Wolff, K.; Stingl, G.

In: Journal of Immunology, Vol. 137, No. 1, 10.09.1986, p. 155-159.

Research output: Contribution to journalArticle

Steiner, G, Tschachler, E, Tani, M, Malek, T, Shevach, EM, Holter, W, Knapp, W, Wolff, K & Stingl, G 1986, 'Interleukin 2 receptors on cultured murine epidermal Langerhans cells', Journal of Immunology, vol. 137, no. 1, pp. 155-159.
Steiner G, Tschachler E, Tani M, Malek T, Shevach EM, Holter W et al. Interleukin 2 receptors on cultured murine epidermal Langerhans cells. Journal of Immunology. 1986 Sep 10;137(1):155-159.
Steiner, G. ; Tschachler, E. ; Tani, M. ; Malek, Thomas ; Shevach, E. M. ; Holter, W. ; Knapp, W. ; Wolff, K. ; Stingl, G. / Interleukin 2 receptors on cultured murine epidermal Langerhans cells. In: Journal of Immunology. 1986 ; Vol. 137, No. 1. pp. 155-159.
@article{b662fb61fdbd435fb9f2411251849c3d,
title = "Interleukin 2 receptors on cultured murine epidermal Langerhans cells",
abstract = "Rat monoclonal antibodies 3C7 and 7D4 detect two distinct functional regions of the murine interleukin 2 (IL 2) receptor. When studying the emergence kinetics of IL 2 receptors in mixed epidermal cell (EC)-lymphocyte cultures by using 3C7 and 7D4 in an indirect immunofluorescence assay, we regularly encountered a distinctive membrane fluorescence not only on lymphocytes, but also on a subpopulation of cells exhibiting a dendritic morphology. Reasoning that these 3C7/7D4-reactive dendritic cells might represent a subpopulation of epidermal dendritic cells, we studied mouse EC for the presence of 3C7/7D4-reactive cells. Although 3C7/7D4 reactivity was never detected on freshly isolated EC or on epidermal sheets, a small number of 3C7/7D4+ cells was encountered after 24 to 48 hr of culture. These cells exhibited a dendritic shape, expressed Ia antigens, lacked Thy-1 antigens, and displayed the ultrastructural features of Langerhans cells (LC) with the notable exception of Birbeck granules. Although after 24 hr, only 20{\%} of Ia+ EC were 3C7/7D4+, the vast majority of LC displayed 3C7/D74 binding sites after 4 to 5 days of culture. Preincubation of cultured LC-enriched EC with recombinant human IL 2 prevented subsequent 3C7-but not 7D4-binding to these cells. Western blot analysis of 7D4-reactive material of detergent extracts from LC-enriched EC revealed three bands in the same m.w. range as reported for CTLL cells. These results demonstrate that cultured LC express IL 2 receptors and may bear important implications for a better understanding of growth regulation, differentiation, and immunologic functions of LC.",
author = "G. Steiner and E. Tschachler and M. Tani and Thomas Malek and Shevach, {E. M.} and W. Holter and W. Knapp and K. Wolff and G. Stingl",
year = "1986",
month = "9",
day = "10",
language = "English",
volume = "137",
pages = "155--159",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "1",

}

TY - JOUR

T1 - Interleukin 2 receptors on cultured murine epidermal Langerhans cells

AU - Steiner, G.

AU - Tschachler, E.

AU - Tani, M.

AU - Malek, Thomas

AU - Shevach, E. M.

AU - Holter, W.

AU - Knapp, W.

AU - Wolff, K.

AU - Stingl, G.

PY - 1986/9/10

Y1 - 1986/9/10

N2 - Rat monoclonal antibodies 3C7 and 7D4 detect two distinct functional regions of the murine interleukin 2 (IL 2) receptor. When studying the emergence kinetics of IL 2 receptors in mixed epidermal cell (EC)-lymphocyte cultures by using 3C7 and 7D4 in an indirect immunofluorescence assay, we regularly encountered a distinctive membrane fluorescence not only on lymphocytes, but also on a subpopulation of cells exhibiting a dendritic morphology. Reasoning that these 3C7/7D4-reactive dendritic cells might represent a subpopulation of epidermal dendritic cells, we studied mouse EC for the presence of 3C7/7D4-reactive cells. Although 3C7/7D4 reactivity was never detected on freshly isolated EC or on epidermal sheets, a small number of 3C7/7D4+ cells was encountered after 24 to 48 hr of culture. These cells exhibited a dendritic shape, expressed Ia antigens, lacked Thy-1 antigens, and displayed the ultrastructural features of Langerhans cells (LC) with the notable exception of Birbeck granules. Although after 24 hr, only 20% of Ia+ EC were 3C7/7D4+, the vast majority of LC displayed 3C7/D74 binding sites after 4 to 5 days of culture. Preincubation of cultured LC-enriched EC with recombinant human IL 2 prevented subsequent 3C7-but not 7D4-binding to these cells. Western blot analysis of 7D4-reactive material of detergent extracts from LC-enriched EC revealed three bands in the same m.w. range as reported for CTLL cells. These results demonstrate that cultured LC express IL 2 receptors and may bear important implications for a better understanding of growth regulation, differentiation, and immunologic functions of LC.

AB - Rat monoclonal antibodies 3C7 and 7D4 detect two distinct functional regions of the murine interleukin 2 (IL 2) receptor. When studying the emergence kinetics of IL 2 receptors in mixed epidermal cell (EC)-lymphocyte cultures by using 3C7 and 7D4 in an indirect immunofluorescence assay, we regularly encountered a distinctive membrane fluorescence not only on lymphocytes, but also on a subpopulation of cells exhibiting a dendritic morphology. Reasoning that these 3C7/7D4-reactive dendritic cells might represent a subpopulation of epidermal dendritic cells, we studied mouse EC for the presence of 3C7/7D4-reactive cells. Although 3C7/7D4 reactivity was never detected on freshly isolated EC or on epidermal sheets, a small number of 3C7/7D4+ cells was encountered after 24 to 48 hr of culture. These cells exhibited a dendritic shape, expressed Ia antigens, lacked Thy-1 antigens, and displayed the ultrastructural features of Langerhans cells (LC) with the notable exception of Birbeck granules. Although after 24 hr, only 20% of Ia+ EC were 3C7/7D4+, the vast majority of LC displayed 3C7/D74 binding sites after 4 to 5 days of culture. Preincubation of cultured LC-enriched EC with recombinant human IL 2 prevented subsequent 3C7-but not 7D4-binding to these cells. Western blot analysis of 7D4-reactive material of detergent extracts from LC-enriched EC revealed three bands in the same m.w. range as reported for CTLL cells. These results demonstrate that cultured LC express IL 2 receptors and may bear important implications for a better understanding of growth regulation, differentiation, and immunologic functions of LC.

UR - http://www.scopus.com/inward/record.url?scp=0022575560&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022575560&partnerID=8YFLogxK

M3 - Article

VL - 137

SP - 155

EP - 159

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 1

ER -