Interleukin 2 receptor dysfunction in mice undergoing a graft-vs-host reaction

L. J. Joseph, T. Iwasaki, T. R. Malek, G. M. Shearer

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Mice of the (C57BL/10xB10.A)F1 combination were given a single i.v. inoculation of 3 to 4 x 107 B10.A spleen cells, which induces a graft-vs-host (GVH)-associated immune deficiency in F1 mice. Between 1 and 4 wk later, spleen cells from the F1 mice were tested for the expression of IL 2 receptors by flow microfluorometry, using the 7D4 rat monoclonal antibody directed against an epitope murine IL 2 receptor. A reduction in intensity of spleen cell staining with 7D4 was detected as early as 8 days after parental cell inoculation, and no IL 2 receptors were detected by 28 days after initiation of GVH. Furthermore, the loss of IL 2 receptors was correlated with abrogation of proliferative responses to concanavalin A and lipopolysaccharide, of IL 2 production, and of cytotoxic T lymphocyte responses. These observations may be relevant for our understanding of GVH reactions, of immune disorders associated with GVB, and possibly of primary and acquired immunodeficiencies in general.

Original languageEnglish (US)
Pages (from-to)1846-1850
Number of pages5
JournalJournal of Immunology
Volume135
Issue number3
StatePublished - Dec 1 1985
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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