Interleukin 2 induces antigen-reactive T cell lines to secrete BCGF-I

Maureen Howard, Louis Matis, Thomas R. Malek, Ethan Shevach, Wayne Kell, David Cohen, Kenji Nakanishi, William E. Paul

Research output: Contribution to journalArticle

90 Scopus citations

Abstract

Antigen-activated T lymphocytes produce within 24 h of stimulation a factor that is distinguishable biochemically and functionally from the B cell costimulating growth factor, BCGF-I, originally identified in induced EL4 supernatants: Supernatants from antigen-stimulated T cell lines are not directly mitogenic for resting B cells, but synergize in an H-2-unrestricted manner with anti-Ig activated B cells to produce polyclonal proliferation but not antibody-forming-cell development; biochemical studies reveal the B cell co-stimulating factor present in antigen-stimulated T cell line supernatants is identical by phenyl Sepharose chromatography and isoelectric focusing (IEF) to EL4 supernatant BCGF-I. We thus conclude that normal T cells produce BCGF-I in response to antigenic stimulation. Analysis of the mechanism of BCGF-I production by antigen-stimulated T cells showed that optimum amounts of BCGF-I were obtained as quickly as 24 h post-stimulation, and that the factor producing cells in the T cell line investigated bore the Lyt-1+2- phenotype. As few as 106 T cells produced sufficient BCGF-I to support the proliferation of 5 x 104 purified anti-Ig activated T cells. Finally, the activation of normal T cell lines to produce BCGF-I required either antigen presented in the context of syngeneic antigen-presenting cells (APC) or interleukin 2 (IL-2).

Original languageEnglish (US)
Pages (from-to)2024-2039
Number of pages16
JournalJournal of Experimental Medicine
Volume158
Issue number6
DOIs
StatePublished - Dec 1 1983

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Howard, M., Matis, L., Malek, T. R., Shevach, E., Kell, W., Cohen, D., Nakanishi, K., & Paul, W. E. (1983). Interleukin 2 induces antigen-reactive T cell lines to secrete BCGF-I. Journal of Experimental Medicine, 158(6), 2024-2039. https://doi.org/10.1084/jem.158.6.2024