TY - JOUR
T1 - Interleukin-2 and Inflammation Induce Distinct Transcriptional Programs that Promote the Differentiation of Effector Cytolytic T Cells
AU - Pipkin, Matthew E.
AU - Sacks, Jilian A.
AU - Cruz-Guilloty, Fernando
AU - Lichtenheld, Mathias G.
AU - Bevan, Michael J.
AU - Rao, Anjana
N1 - Funding Information:
We thank Dr. S. Reiner for generously providing the Eomes-VP16 retroviral construct. This work was funded by NIH grants AI44432 and AI707088 (to A.R.) and AI19335 (to M.J.B.); University of Miami Developmental Center for AIDS Research 5P30AI073961 (to M.G.L.); and the National Cancer Institute F32CA126247 (to M.E.P). F.C.G. was a predoctoral fellow of the Ryan Foundation and was supported by a Ford Foundation Predoctoral Fellowship.
PY - 2010/1/29
Y1 - 2010/1/29
N2 - Interleukin(IL)-2 and inflammation regulate effector and memory cytolytic T-lymphocyte (CTL) generation during infection. We demonstrate a complex interplay between IL-2 and inflammatory signals during CTL differentiation. IL-2 stimulation induced the transcription factor eomesodermin (Eomes), upregulated perforin (Prf1) transcription, and repressed re-expression of memory CTL markers Bcl6 and IL-7Rα. Binding of Eomes and STAT5 to Prf1 cis-regulatory regions correlated with transcriptional initiation (increased recruitment of RNA polymerase II to the Prf1 promoter). Inflammation (CpG, IL-12) enhanced expression of IL-2Rα and the transcription factor T-bet, but countered late Eomes and perforin induction while preventing IL-7Rα repression by IL-2. After infection of mice with lymphocytic choriomeningitis virus, IL-2Rα-deficient effector CD8+ T cells expressed more Bcl6 but less perforin and granzyme B, formed fewer KLRG-1+ and T-bet-expressing CTL, and killed poorly. Thus, inflammation influences both effector and memory CTL differentiation, whereas persistent IL-2 stimulation promotes effector at the expense of memory CTL development.
AB - Interleukin(IL)-2 and inflammation regulate effector and memory cytolytic T-lymphocyte (CTL) generation during infection. We demonstrate a complex interplay between IL-2 and inflammatory signals during CTL differentiation. IL-2 stimulation induced the transcription factor eomesodermin (Eomes), upregulated perforin (Prf1) transcription, and repressed re-expression of memory CTL markers Bcl6 and IL-7Rα. Binding of Eomes and STAT5 to Prf1 cis-regulatory regions correlated with transcriptional initiation (increased recruitment of RNA polymerase II to the Prf1 promoter). Inflammation (CpG, IL-12) enhanced expression of IL-2Rα and the transcription factor T-bet, but countered late Eomes and perforin induction while preventing IL-7Rα repression by IL-2. After infection of mice with lymphocytic choriomeningitis virus, IL-2Rα-deficient effector CD8+ T cells expressed more Bcl6 but less perforin and granzyme B, formed fewer KLRG-1+ and T-bet-expressing CTL, and killed poorly. Thus, inflammation influences both effector and memory CTL differentiation, whereas persistent IL-2 stimulation promotes effector at the expense of memory CTL development.
KW - CELLIMMUNO
KW - MOLIMMUNO
UR - http://www.scopus.com/inward/record.url?scp=74549216531&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=74549216531&partnerID=8YFLogxK
U2 - 10.1016/j.immuni.2009.11.012
DO - 10.1016/j.immuni.2009.11.012
M3 - Article
C2 - 20096607
AN - SCOPUS:74549216531
VL - 32
SP - 79
EP - 90
JO - Immunity
JF - Immunity
SN - 1074-7613
IS - 1
ER -
