Interleukin-17A

A T-cell-derived growth factor for murine and human mesenchymal stem cells

Weitao Huang, Vincent La Russa, Azam Alzoubi, Paul Schwarzenberger

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Interleukin-17A (IL-17A) is a proinflammatory cytokine expressed in activated T-cells. It is required for microbial host defense and is a potent stimulator of granulopoiesis. In a dose-dependent fashion, IL-17A expanded human mesenchymal stem cells (MSCs) and induced the proliferation of mature stroma cells in bone marrow-derived stroma cultures. Recombinant human interleukin-17A (rhIL-17A) nearly doubled colony-forming unit-fibroblast (CFU-f) frequency and almost tripled the surface area covered by stroma. In a murine transplant model, in vivo murine (m)IL-17A expression enhanced CFU-f by 2.5-fold. Enrichment of the graft with CD4+ T-cell resulted in a 7.5-fold increase in CFU-f in normal C57BL/6, but only threefold in IL-17Ra-/- mice on day 14 post-transplant. In this transplant model, in vivo blockade of IL-17A in C57BL/6 mice resembled the phenotype of IL-17Ra-/- mice. Approximately half of the T-cell-mediated effect on MSC recovery following radiation-conditioned transplantation was attributed to the IL-17A/IL-17Ra pathway. Pluripotent MSCs have the potential of regenerating various tissues, and mature stroma cells are critical elements of the hematopoietic microenvironment (HME). The HME is pivotal for formation and maintenance of functional blood cells. As a newly identified stroma cell growth factor, IL-17A might have potential applications for novel treatment approaches involving MSCs, such as tissue graft engineering.

Original languageEnglish
Pages (from-to)1512-1518
Number of pages7
JournalStem Cells
Volume24
Issue number6
DOIs
StatePublished - Jun 1 2006

Fingerprint

Interleukin-17
Mesenchymal Stromal Cells
Interleukin-2
Transplants
Stem Cells
Fibroblasts
T-Lymphocytes
Pluripotent Stem Cells
Tissue Engineering
Inbred C57BL Mouse
Bone Marrow Cells
Blood Cells
Intercellular Signaling Peptides and Proteins
Transplantation
Maintenance
Cell Proliferation
Radiation
Cytokines
Phenotype

Keywords

  • Hematopoeitic microenvironment
  • Interleukin-17
  • Mesenchymal stem cells
  • Stroma
  • T-cells

ASJC Scopus subject areas

  • Cell Biology

Cite this

Interleukin-17A : A T-cell-derived growth factor for murine and human mesenchymal stem cells. / Huang, Weitao; La Russa, Vincent; Alzoubi, Azam; Schwarzenberger, Paul.

In: Stem Cells, Vol. 24, No. 6, 01.06.2006, p. 1512-1518.

Research output: Contribution to journalArticle

Huang, W, La Russa, V, Alzoubi, A & Schwarzenberger, P 2006, 'Interleukin-17A: A T-cell-derived growth factor for murine and human mesenchymal stem cells', Stem Cells, vol. 24, no. 6, pp. 1512-1518. https://doi.org/10.1634/stemcells.2005-0156
Huang, Weitao ; La Russa, Vincent ; Alzoubi, Azam ; Schwarzenberger, Paul. / Interleukin-17A : A T-cell-derived growth factor for murine and human mesenchymal stem cells. In: Stem Cells. 2006 ; Vol. 24, No. 6. pp. 1512-1518.
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