Interleukin 1 protects hair follicles from cytarabine (ARA-C)-induced toxicity in vivo and in vitro

Joaquin J Jimenez, Marty E. Sawaya, Adel A Yunis

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

ImuVert, a biologic response modifier, and interleukin 1 (IL 1) have been shown to protect the young rat from alopecia induced by cytarabine (ARA-C). In the present study the inhibition by ARA-C of DNA synthesis in hair follicles (HFs) and the protective effect of ImuVert and IL 1 were investigated in vivo and in vitro. Both ImuVert and IL 1 were equally effective in protecting rats from ARA-C-induced alopecia. DNA synthesis in HFs isolated from ARA-C-treated animals was 10-20% of untreated controls. Follicles isolated from animals given either ImuVert or IL 1 before ARA-C exhibited normal DNA synthesis. In vitro, the incubation of normal rat HF with ARA-C resulted in 80% inhibition of [3H]-thymidine uptake. Preincubation of the follicles for 1 hr with IL 1 before the addition of ARA-C completely blocked the inhibition. Preincubation with imuVert, however, was less effective in blocking the inhibition from ARA-C.

Original languageEnglish
Pages (from-to)911-913
Number of pages3
JournalFASEB Journal
Volume6
Issue number3
StatePublished - Feb 1 1992

Fingerprint

hair follicles
Hair Follicle
Cytarabine
interleukin-1
Interleukin-1
Toxicity
toxicity
Rats
alopecia
Alopecia
synthesis
DNA
rats
Animals
modifiers (genes)
thymidine
Thymidine
protective effect
animals
ImuVert

Keywords

  • Alopecia
  • Chemotherapy
  • DNA synthesis
  • Hair follicles
  • I1 1
  • ImuVert

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Interleukin 1 protects hair follicles from cytarabine (ARA-C)-induced toxicity in vivo and in vitro. / Jimenez, Joaquin J; Sawaya, Marty E.; Yunis, Adel A.

In: FASEB Journal, Vol. 6, No. 3, 01.02.1992, p. 911-913.

Research output: Contribution to journalArticle

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N2 - ImuVert, a biologic response modifier, and interleukin 1 (IL 1) have been shown to protect the young rat from alopecia induced by cytarabine (ARA-C). In the present study the inhibition by ARA-C of DNA synthesis in hair follicles (HFs) and the protective effect of ImuVert and IL 1 were investigated in vivo and in vitro. Both ImuVert and IL 1 were equally effective in protecting rats from ARA-C-induced alopecia. DNA synthesis in HFs isolated from ARA-C-treated animals was 10-20% of untreated controls. Follicles isolated from animals given either ImuVert or IL 1 before ARA-C exhibited normal DNA synthesis. In vitro, the incubation of normal rat HF with ARA-C resulted in 80% inhibition of [3H]-thymidine uptake. Preincubation of the follicles for 1 hr with IL 1 before the addition of ARA-C completely blocked the inhibition. Preincubation with imuVert, however, was less effective in blocking the inhibition from ARA-C.

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