Interleukin-1β messenger ribonucleic acid and protein levels after fluid-percussion brain injury in rats: Importance of injury severity and brain temperature

Kosaku Kinoshita, Katina Chatzipanteli, Elizabeth Vitarbo, Jessie S. Truettner, Ofelia F. Alonso, W. Dalton Dietrich, Warren R. Selman, Stefan M. Lee, Daniel F. Kelly

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

OBJECTIVE: Posttraumatic temperature manipulations have been reported to significantly influence the inflammatory response to traumatic brain injury (TBI). The purpose of this study was to determine the temporal and regional profiles of messenger ribonucleic acid (mRNA) expression and protein levels for the proinflammatory cytokine interleukin-1β (IL-1β, after moderate or severe TBI. The effects of posttraumatic hypothermia (33°C) or hyperthermia (39.5°C) on these consequences of TBI were then determined. METHODS: Male Sprague-Dawley rats underwent fluid-percussion brain injury. In the first phase of the study, rats were killed 15 minutes or 1, 3, or 24 hours after moderate TBI (1.8-2.2 atmospheres), for reverse transcription-polymerase chain reaction analysis. Other groups of rats were killed 1, 3, 24, or 72 hours after moderate or severe TBI (2.4-2.7 atmospheres), for protein analysis. In the second phase, rats underwent moderate fluid-percussion brain injury, followed immediately by 3 hours of posttraumatic normothermia (37°C), hyperthermia (39.5°C), or hypothermia (33°C), and were then killed, for analyses of protein levels and mRNA expression. Brain samples, including cerebral cortex, hippocampus, thalamus, and cerebellum, were dissected and stored at -80°C until analyzed. RESULTS: The findings indicated that mRNA levels were increased (P < 0.05) as early as 1 hour after TBI and remained elevated up to 3 hours after moderate TBI. Although both moderate and severe TBI induced increased levels of IL-1β (P < 0.05), increased protein levels were also noted in remote brain structures after severe TBI. Posttraumatic hypothermia attenuated IL-1β protein levels, compared with normothermia (P < 0.05), although the levels remained elevated in comparison with sham values. In contrast, hyperthermia had no significant effect on IL-1β levels, compared with normothermic values. Posttraumatic temperature manipulations had no significant effect on IL-1β mRNA levels. CONCLUSION: Injury severity determines the degree of IL-1β protein level elevation after TBI. The effects of posttraumatic hypothermia on IL-1β protein levels (an important mediator of neurodegeneration after TBI) may partly explain the established effects of posttraumatic temperature manipulations on inflammatory processes after TBI.

Original languageEnglish
Pages (from-to)195-203
Number of pages9
JournalNeurosurgery
Volume51
Issue number1
DOIs
StatePublished - Jul 1 2002

Fingerprint

Percussion
Interleukin-1
Brain Injuries
RNA
Temperature
Proteins
Hypothermia
Fever
Atmosphere
Traumatic Brain Injury
Brain
Thalamus
Cerebral Cortex
Cerebellum
Reverse Transcription
Sprague Dawley Rats
Hippocampus

Keywords

  • Hyperthermia
  • Hypothermia
  • Inflammation and secondary injury
  • Interleukin-1β
  • Traumatic brain injury

ASJC Scopus subject areas

  • Clinical Neurology
  • Surgery

Cite this

Interleukin-1β messenger ribonucleic acid and protein levels after fluid-percussion brain injury in rats : Importance of injury severity and brain temperature. / Kinoshita, Kosaku; Chatzipanteli, Katina; Vitarbo, Elizabeth; Truettner, Jessie S.; Alonso, Ofelia F.; Dalton Dietrich, W.; Selman, Warren R.; Lee, Stefan M.; Kelly, Daniel F.

In: Neurosurgery, Vol. 51, No. 1, 01.07.2002, p. 195-203.

Research output: Contribution to journalArticle

Kinoshita, Kosaku ; Chatzipanteli, Katina ; Vitarbo, Elizabeth ; Truettner, Jessie S. ; Alonso, Ofelia F. ; Dalton Dietrich, W. ; Selman, Warren R. ; Lee, Stefan M. ; Kelly, Daniel F. / Interleukin-1β messenger ribonucleic acid and protein levels after fluid-percussion brain injury in rats : Importance of injury severity and brain temperature. In: Neurosurgery. 2002 ; Vol. 51, No. 1. pp. 195-203.
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abstract = "OBJECTIVE: Posttraumatic temperature manipulations have been reported to significantly influence the inflammatory response to traumatic brain injury (TBI). The purpose of this study was to determine the temporal and regional profiles of messenger ribonucleic acid (mRNA) expression and protein levels for the proinflammatory cytokine interleukin-1β (IL-1β, after moderate or severe TBI. The effects of posttraumatic hypothermia (33°C) or hyperthermia (39.5°C) on these consequences of TBI were then determined. METHODS: Male Sprague-Dawley rats underwent fluid-percussion brain injury. In the first phase of the study, rats were killed 15 minutes or 1, 3, or 24 hours after moderate TBI (1.8-2.2 atmospheres), for reverse transcription-polymerase chain reaction analysis. Other groups of rats were killed 1, 3, 24, or 72 hours after moderate or severe TBI (2.4-2.7 atmospheres), for protein analysis. In the second phase, rats underwent moderate fluid-percussion brain injury, followed immediately by 3 hours of posttraumatic normothermia (37°C), hyperthermia (39.5°C), or hypothermia (33°C), and were then killed, for analyses of protein levels and mRNA expression. Brain samples, including cerebral cortex, hippocampus, thalamus, and cerebellum, were dissected and stored at -80°C until analyzed. RESULTS: The findings indicated that mRNA levels were increased (P < 0.05) as early as 1 hour after TBI and remained elevated up to 3 hours after moderate TBI. Although both moderate and severe TBI induced increased levels of IL-1β (P < 0.05), increased protein levels were also noted in remote brain structures after severe TBI. Posttraumatic hypothermia attenuated IL-1β protein levels, compared with normothermia (P < 0.05), although the levels remained elevated in comparison with sham values. In contrast, hyperthermia had no significant effect on IL-1β levels, compared with normothermic values. Posttraumatic temperature manipulations had no significant effect on IL-1β mRNA levels. CONCLUSION: Injury severity determines the degree of IL-1β protein level elevation after TBI. The effects of posttraumatic hypothermia on IL-1β protein levels (an important mediator of neurodegeneration after TBI) may partly explain the established effects of posttraumatic temperature manipulations on inflammatory processes after TBI.",
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AU - Chatzipanteli, Katina

AU - Vitarbo, Elizabeth

AU - Truettner, Jessie S.

AU - Alonso, Ofelia F.

AU - Dalton Dietrich, W.

AU - Selman, Warren R.

AU - Lee, Stefan M.

AU - Kelly, Daniel F.

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N2 - OBJECTIVE: Posttraumatic temperature manipulations have been reported to significantly influence the inflammatory response to traumatic brain injury (TBI). The purpose of this study was to determine the temporal and regional profiles of messenger ribonucleic acid (mRNA) expression and protein levels for the proinflammatory cytokine interleukin-1β (IL-1β, after moderate or severe TBI. The effects of posttraumatic hypothermia (33°C) or hyperthermia (39.5°C) on these consequences of TBI were then determined. METHODS: Male Sprague-Dawley rats underwent fluid-percussion brain injury. In the first phase of the study, rats were killed 15 minutes or 1, 3, or 24 hours after moderate TBI (1.8-2.2 atmospheres), for reverse transcription-polymerase chain reaction analysis. Other groups of rats were killed 1, 3, 24, or 72 hours after moderate or severe TBI (2.4-2.7 atmospheres), for protein analysis. In the second phase, rats underwent moderate fluid-percussion brain injury, followed immediately by 3 hours of posttraumatic normothermia (37°C), hyperthermia (39.5°C), or hypothermia (33°C), and were then killed, for analyses of protein levels and mRNA expression. Brain samples, including cerebral cortex, hippocampus, thalamus, and cerebellum, were dissected and stored at -80°C until analyzed. RESULTS: The findings indicated that mRNA levels were increased (P < 0.05) as early as 1 hour after TBI and remained elevated up to 3 hours after moderate TBI. Although both moderate and severe TBI induced increased levels of IL-1β (P < 0.05), increased protein levels were also noted in remote brain structures after severe TBI. Posttraumatic hypothermia attenuated IL-1β protein levels, compared with normothermia (P < 0.05), although the levels remained elevated in comparison with sham values. In contrast, hyperthermia had no significant effect on IL-1β levels, compared with normothermic values. Posttraumatic temperature manipulations had no significant effect on IL-1β mRNA levels. CONCLUSION: Injury severity determines the degree of IL-1β protein level elevation after TBI. The effects of posttraumatic hypothermia on IL-1β protein levels (an important mediator of neurodegeneration after TBI) may partly explain the established effects of posttraumatic temperature manipulations on inflammatory processes after TBI.

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