Interferon-gamma gene expression during acute graft-versus-host disease: Relationship to MHC induction and tissue injury

Nollaig A. Parfrey, Amr El-Sheikh, Elizabeth A. Monckton, Sandra M. Cockfield, Philip F. Halloran, Elina Linetsky

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The pathogenetic role of interferon-γ (IFN-γ) in acute graft-versus- host disease (GVHD) was examined in a murine model. IFN-γ gene expression was evaluated by northern blotting and mRNA in situ hybridization. The temporal and tissue specific patterns of IFN-γ gene expression were related to the patterns of major histocompatibility complex (MHC) antigen induction and of tissue injury. Markedly increased levels of IFN-γ transcripts were seen in the spleen during the early lymphoproliferative phase and coincided with widespread MHC induction in non-lymphoid tissues. Increased IFN-γ transcripts were also found in the non-lymphoid target tissues during the phase of subsequent tissue injury. These findings support a role for IFN-γ in leading to widespread MHC induction during acute GVHD and suggest that IFN-γ may also contribute to target tissue injury during acute GVHD.

Original languageEnglish (US)
Pages (from-to)99-104
Number of pages6
JournalJournal of Pathology
Volume189
Issue number1
DOIs
StatePublished - Sep 6 1999
Externally publishedYes

Fingerprint

Graft vs Host Disease
Major Histocompatibility Complex
Interferons
Interferon-gamma
Gene Expression
Wounds and Injuries
Histocompatibility Antigens
Northern Blotting
In Situ Hybridization
Spleen
Messenger RNA

Keywords

  • Gene expression
  • Graft-versus-host disease
  • Interferon-γ
  • Major histocompatibility complex
  • Tissue injury

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Interferon-gamma gene expression during acute graft-versus-host disease : Relationship to MHC induction and tissue injury. / Parfrey, Nollaig A.; El-Sheikh, Amr; Monckton, Elizabeth A.; Cockfield, Sandra M.; Halloran, Philip F.; Linetsky, Elina.

In: Journal of Pathology, Vol. 189, No. 1, 06.09.1999, p. 99-104.

Research output: Contribution to journalArticle

Parfrey, Nollaig A. ; El-Sheikh, Amr ; Monckton, Elizabeth A. ; Cockfield, Sandra M. ; Halloran, Philip F. ; Linetsky, Elina. / Interferon-gamma gene expression during acute graft-versus-host disease : Relationship to MHC induction and tissue injury. In: Journal of Pathology. 1999 ; Vol. 189, No. 1. pp. 99-104.
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