Abstract
The pathogenetic role of interferon-γ (IFN-γ) in acute graft-versus- host disease (GVHD) was examined in a murine model. IFN-γ gene expression was evaluated by northern blotting and mRNA in situ hybridization. The temporal and tissue specific patterns of IFN-γ gene expression were related to the patterns of major histocompatibility complex (MHC) antigen induction and of tissue injury. Markedly increased levels of IFN-γ transcripts were seen in the spleen during the early lymphoproliferative phase and coincided with widespread MHC induction in non-lymphoid tissues. Increased IFN-γ transcripts were also found in the non-lymphoid target tissues during the phase of subsequent tissue injury. These findings support a role for IFN-γ in leading to widespread MHC induction during acute GVHD and suggest that IFN-γ may also contribute to target tissue injury during acute GVHD.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 99-104 |
| Number of pages | 6 |
| Journal | Journal of Pathology |
| Volume | 189 |
| Issue number | 1 |
| DOIs | |
| State | Published - Sep 6 1999 |
| Externally published | Yes |
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Keywords
- Gene expression
- Graft-versus-host disease
- Interferon-γ
- Major histocompatibility complex
- Tissue injury
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
Interferon-gamma gene expression during acute graft-versus-host disease : Relationship to MHC induction and tissue injury. / Parfrey, Nollaig A.; El-Sheikh, Amr; Monckton, Elizabeth A.; Cockfield, Sandra M.; Halloran, Philip F.; Linetsky, Elina.
In: Journal of Pathology, Vol. 189, No. 1, 06.09.1999, p. 99-104.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Interferon-gamma gene expression during acute graft-versus-host disease
T2 - Relationship to MHC induction and tissue injury
AU - Parfrey, Nollaig A.
AU - El-Sheikh, Amr
AU - Monckton, Elizabeth A.
AU - Cockfield, Sandra M.
AU - Halloran, Philip F.
AU - Linetsky, Elina
PY - 1999/9/6
Y1 - 1999/9/6
N2 - The pathogenetic role of interferon-γ (IFN-γ) in acute graft-versus- host disease (GVHD) was examined in a murine model. IFN-γ gene expression was evaluated by northern blotting and mRNA in situ hybridization. The temporal and tissue specific patterns of IFN-γ gene expression were related to the patterns of major histocompatibility complex (MHC) antigen induction and of tissue injury. Markedly increased levels of IFN-γ transcripts were seen in the spleen during the early lymphoproliferative phase and coincided with widespread MHC induction in non-lymphoid tissues. Increased IFN-γ transcripts were also found in the non-lymphoid target tissues during the phase of subsequent tissue injury. These findings support a role for IFN-γ in leading to widespread MHC induction during acute GVHD and suggest that IFN-γ may also contribute to target tissue injury during acute GVHD.
AB - The pathogenetic role of interferon-γ (IFN-γ) in acute graft-versus- host disease (GVHD) was examined in a murine model. IFN-γ gene expression was evaluated by northern blotting and mRNA in situ hybridization. The temporal and tissue specific patterns of IFN-γ gene expression were related to the patterns of major histocompatibility complex (MHC) antigen induction and of tissue injury. Markedly increased levels of IFN-γ transcripts were seen in the spleen during the early lymphoproliferative phase and coincided with widespread MHC induction in non-lymphoid tissues. Increased IFN-γ transcripts were also found in the non-lymphoid target tissues during the phase of subsequent tissue injury. These findings support a role for IFN-γ in leading to widespread MHC induction during acute GVHD and suggest that IFN-γ may also contribute to target tissue injury during acute GVHD.
KW - Gene expression
KW - Graft-versus-host disease
KW - Interferon-γ
KW - Major histocompatibility complex
KW - Tissue injury
UR - http://www.scopus.com/inward/record.url?scp=0032867745&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032867745&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1096-9896(199909)189:1<99::AID-PATH404>3.0.CO;2-F
DO - 10.1002/(SICI)1096-9896(199909)189:1<99::AID-PATH404>3.0.CO;2-F
M3 - Article
C2 - 10451495
AN - SCOPUS:0032867745
VL - 189
SP - 99
EP - 104
JO - Journal of Pathology
JF - Journal of Pathology
SN - 0022-3417
IS - 1
ER -