Interfacial behavior of Proteinase K enzyme at air-saline subphase

Suraj Paudyal, Ganesh Sigdel, Sujit K. Shah, Shiv K. Sharma, John D. Grubb, Miodrag Micic, Luciano Caseli, Roger M. Leblanc

Research output: Contribution to journalArticlepeer-review


This study investigates the interfacial behavior of the proteinase K enzyme at air–water interface. Adsorption of enzyme on the surface was induced using saline subphase. The surface packing and stability of the enzyme was investigated using of surface pressure-area (π-A) and surface potential-area (ΔV-A) isotherms. Proteinase K enzyme forms film at air-aqueous interface and demonstrates good stability as shown through compression-decompression cycle experiments. To characterize the surface assembly morphology of the interfacial enzymes UV–vis and fluorescence spectroscopic techniques were used. The data revealed that the enzyme Langmuir monolayer has good homogeneity with no evidence of aggregates during compression. The secondary structure of the enzyme at interface was determined to be α-helix using p-polarized infrared-reflection absorption spectroscopy. This was confirmed through Circular dichroism spectra of the enzyme Langmuir-Blodgett (LB) film which showed that the major conformation present were α-helices.

Original languageEnglish (US)
Pages (from-to)701-708
Number of pages8
JournalJournal of Colloid And Interface Science
StatePublished - Jun 15 2022
Externally publishedYes


  • Circular dichroism
  • Infrared-reflection absorption spectroscopy
  • Langmuir monolayer
  • Langmuir-Blodgett
  • Proteinase K

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Surfaces, Coatings and Films
  • Colloid and Surface Chemistry


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