Interactive effects of copper and dissolved organic matter on sodium uptake, copper bioaccumulation, and oxidative stress in juvenile freshwater mussels (Lampsilis siliquoidea)

Marina Giacomin, Patricia L. Gillis, Adalto Bianchini, Chris M. Wood

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Freshwater mussels are exceptionally sensitive to many contaminants including metals, but the mechanisms of toxicity are not fully understood. Similarly, our understanding of the protective effects of dissolved organic matter (DOM) is also undergoing revision, since recent studies have found that DOM may also directly affect organism physiology, in addition to its well known capability in complexing and reducing bioavailability of metals. In the present study, these issues were investigated in juvenile (6-12 months old) freshwater mussels (Lampsilis siliquoidea) in moderately-hard reconstituted water (Ca2+=0.406mmol/L; Mg2+=0.537mmol/L; Na+=1.261mmol/L;K+=0.077mmol/L; hardness=80-100mg/L CaCO3; pH=8.02 and DOM=0.3mgC/L). Mussels were acutely exposed (24 and 96h) to Cu (0, 2 or 12μgCu/L) combined with three concentrations (0, 3 or 6mgC/L) of DOM of terrigenous origin (Luther Marsh). We analyzed unidirectional Na+ influx, whole-body ion content (Na+, K+, Ca2+ and Mg2+), enzyme (Na+/K+-ATPase, H+-ATPase and carbonic anhydrase) activities, copper bioaccumulation and oxidative stress-related parameters. Exposure to DOM alone caused a marked increase in the unidirectional Na+ influx rate and a decrease in v-type H+-ATPase activity, suggesting that DOM alone can cause alterations in membrane transport functions and therefore, whole-body Na+ metabolism. Unidirectional Na+ influx rate and Na+K+-ATPase activity were inhibited when mussels were exposed to the higher Cu concentration tested (12μg Cu/L). The influx inhibition was ameliorated by the simultaneous presence of DOM. At this same Cu concentration, DOM also significantly protected mussels against whole-body Na+ and K+ losses associated with Cu exposure, as well as against Cu bioaccumulation. Oxidative stress parameters did not show clear trends across treatments. Overall, our results indicate that Cu is a potent ionoregulatory toxicant to freshwater mussels. They also demonstrate that natural DOM protects against both Cu bioaccumulation and ionoregulatory toxicity, and that at least part of this protection results from direct positive effects of DOM on Na+ metabolism.

Original languageEnglish
Pages (from-to)105-115
Number of pages11
JournalAquatic Toxicology
Volume144-145
DOIs
StatePublished - Nov 15 2013

Fingerprint

Bivalvia
bioaccumulation
dissolved organic matter
Fresh Water
mussels
Copper
Oxidative Stress
oxidative stress
copper
Sodium
sodium
uptake mechanisms
Proton-Translocating ATPases
Metals
H-transporting ATP synthase
sodium-potassium-exchanging ATPase
Carbonic Anhydrases
Wetlands
Hardness
Biological Availability

Keywords

  • Acute toxicity
  • Copper
  • Dissolved organic matter
  • Freshwater mussel
  • Ionoregulation
  • Oxidative stress

ASJC Scopus subject areas

  • Aquatic Science
  • Health, Toxicology and Mutagenesis

Cite this

Interactive effects of copper and dissolved organic matter on sodium uptake, copper bioaccumulation, and oxidative stress in juvenile freshwater mussels (Lampsilis siliquoidea). / Giacomin, Marina; Gillis, Patricia L.; Bianchini, Adalto; Wood, Chris M.

In: Aquatic Toxicology, Vol. 144-145, 15.11.2013, p. 105-115.

Research output: Contribution to journalArticle

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