Interactions of the p53 protein family in cellular stress response in gastrointestinal tumors

Anna E. Vilgelm, Mary K. Washington, Jinxiong Wei, Heidi Chen, Vladimir S. Prassolov, Alexander Zaika

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

p53, p63, and p73 are members of the p53 protein family involved in regulation of cell cycle, apoptosis, differentiation, and other critical cellular processes. Here, we investigated the contribution of the entire p53 family in chemotherapeutic drug response in gastrointestinal tumors. Real-time PCR and immunohistochemistry revealed complexity and variability of expression profiles of the p53 protein family. Using colon and esophageal cancer cells, we found that the integral transcription activity of the entire p53 family, as measured by the reporter analysis, associated with response to drug treatment in studied cells.We also found that p53 and p73, as well as p63 and p73, bind simultaneously to the promoters of p53 target genes. Taken together, our results support the view that the p53 protein family functions as an interacting network of proteins and show that cellular responses to chemotherapeutic drug treatment are determined by the total activity of the entire p53 family rather than p53 alone.

Original languageEnglish (US)
Pages (from-to)693-705
Number of pages13
JournalMolecular Cancer Therapeutics
Volume9
Issue number3
DOIs
StatePublished - Mar 1 2010
Externally publishedYes

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Neoplasms
Proteins
Pharmaceutical Preparations
p53 Genes
Esophageal Neoplasms
Colonic Neoplasms
Real-Time Polymerase Chain Reaction
Cell Cycle
Immunohistochemistry
Apoptosis
Therapeutics

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Interactions of the p53 protein family in cellular stress response in gastrointestinal tumors. / Vilgelm, Anna E.; Washington, Mary K.; Wei, Jinxiong; Chen, Heidi; Prassolov, Vladimir S.; Zaika, Alexander.

In: Molecular Cancer Therapeutics, Vol. 9, No. 3, 01.03.2010, p. 693-705.

Research output: Contribution to journalArticle

Vilgelm, Anna E. ; Washington, Mary K. ; Wei, Jinxiong ; Chen, Heidi ; Prassolov, Vladimir S. ; Zaika, Alexander. / Interactions of the p53 protein family in cellular stress response in gastrointestinal tumors. In: Molecular Cancer Therapeutics. 2010 ; Vol. 9, No. 3. pp. 693-705.
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