Abstract
p53, p63, and p73 are members of the p53 protein family involved in regulation of cell cycle, apoptosis, differentiation, and other critical cellular processes. Here, we investigated the contribution of the entire p53 family in chemotherapeutic drug response in gastrointestinal tumors. Real-time PCR and immunohistochemistry revealed complexity and variability of expression profiles of the p53 protein family. Using colon and esophageal cancer cells, we found that the integral transcription activity of the entire p53 family, as measured by the reporter analysis, associated with response to drug treatment in studied cells.We also found that p53 and p73, as well as p63 and p73, bind simultaneously to the promoters of p53 target genes. Taken together, our results support the view that the p53 protein family functions as an interacting network of proteins and show that cellular responses to chemotherapeutic drug treatment are determined by the total activity of the entire p53 family rather than p53 alone.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 693-705 |
| Number of pages | 13 |
| Journal | Molecular Cancer Therapeutics |
| Volume | 9 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 1 2010 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Oncology
- Cancer Research
Cite this
Interactions of the p53 protein family in cellular stress response in gastrointestinal tumors. / Vilgelm, Anna E.; Washington, Mary K.; Wei, Jinxiong; Chen, Heidi; Prassolov, Vladimir S.; Zaika, Alexander.
In: Molecular Cancer Therapeutics, Vol. 9, No. 3, 01.03.2010, p. 693-705.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Interactions of the p53 protein family in cellular stress response in gastrointestinal tumors
AU - Vilgelm, Anna E.
AU - Washington, Mary K.
AU - Wei, Jinxiong
AU - Chen, Heidi
AU - Prassolov, Vladimir S.
AU - Zaika, Alexander
PY - 2010/3/1
Y1 - 2010/3/1
N2 - p53, p63, and p73 are members of the p53 protein family involved in regulation of cell cycle, apoptosis, differentiation, and other critical cellular processes. Here, we investigated the contribution of the entire p53 family in chemotherapeutic drug response in gastrointestinal tumors. Real-time PCR and immunohistochemistry revealed complexity and variability of expression profiles of the p53 protein family. Using colon and esophageal cancer cells, we found that the integral transcription activity of the entire p53 family, as measured by the reporter analysis, associated with response to drug treatment in studied cells.We also found that p53 and p73, as well as p63 and p73, bind simultaneously to the promoters of p53 target genes. Taken together, our results support the view that the p53 protein family functions as an interacting network of proteins and show that cellular responses to chemotherapeutic drug treatment are determined by the total activity of the entire p53 family rather than p53 alone.
AB - p53, p63, and p73 are members of the p53 protein family involved in regulation of cell cycle, apoptosis, differentiation, and other critical cellular processes. Here, we investigated the contribution of the entire p53 family in chemotherapeutic drug response in gastrointestinal tumors. Real-time PCR and immunohistochemistry revealed complexity and variability of expression profiles of the p53 protein family. Using colon and esophageal cancer cells, we found that the integral transcription activity of the entire p53 family, as measured by the reporter analysis, associated with response to drug treatment in studied cells.We also found that p53 and p73, as well as p63 and p73, bind simultaneously to the promoters of p53 target genes. Taken together, our results support the view that the p53 protein family functions as an interacting network of proteins and show that cellular responses to chemotherapeutic drug treatment are determined by the total activity of the entire p53 family rather than p53 alone.
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UR - http://www.scopus.com/inward/citedby.url?scp=77949737597&partnerID=8YFLogxK
U2 - 10.1158/1535-7163.MCT-09-0912
DO - 10.1158/1535-7163.MCT-09-0912
M3 - Article
C2 - 20197393
AN - SCOPUS:77949737597
VL - 9
SP - 693
EP - 705
JO - Molecular Cancer Therapeutics
JF - Molecular Cancer Therapeutics
SN - 1535-7163
IS - 3
ER -