Interactions between adipocytes and breast cancer cells stimulate cytokine production and drive Src/Sox2/miR-302b-mediated malignant progression

Manuel Picon-Ruiz, Chendong Pan, Katherine Drews-Elger, Kibeom Jang, Alexandra H. Besser, Dekuang Zhao, Cynthia Morata-Tarifa, Minsoon Kim, Tan Ince, Diana J. Azzam, Seth A. Wander, Bin Wang, Burcu Ergonul, Ram Datar, Richard J Cote, Guy Howard, Dorraya El-Ashry, Pablo Torné-Poyatos, Juan A. Marchal, Joyce M Slingerland

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Abstract

Consequences of the obesity epidemic on cancer morbidity and mortality are not fully appreciated. Obesity is a risk factor for many cancers, but the mechanisms by which it contributes to cancer development and patient outcome have yet to be fully elucidated. Here, we examined the effects of coculturing humanderived adipocytes with established and primary breast cancer cells on tumorigenic potential. We found that the interaction between adipocytes and cancer cells increased the secretion of proinflammatory cytokines. Prolonged culture of cancer cells with adipocytes or cytokines increased the proportion of mammosphereforming cells and of cells expressing stem-like markers in vitro. Furthermore, contact with immature adipocytes increased the abundance of cancer cells with tumor-forming and metastatic potential in vivo. Mechanistic investigations demonstrated that cancer cells cultured with immature adipocytes or cytokines activated Src, thus promoting Sox2, c-Myc, and Nanog upregulation. Moreover, Sox2-dependent induction of miR-302b further stimulated cMYC and SOX2 expression and potentiated the cytokineinduced cancer stem cell-like properties. Finally, we found that Src inhibitors decreased cytokine production after coculture, indicating that Src is not only activated by adipocyte or cytokine exposures, but is also required to sustain cytokine induction. These data support a model in which cancer cell invasion into local fat would establish feed-forward loops to activate Src, maintain proinflammatory cytokine production, and increase tumor-initiating cell abundance and metastatic progression. Collectively, our findings reveal new insights underlying increased breast cancer mortality in obese individuals and provide a novel preclinical rationale to test the efficacy of Src inhibitors for breast cancer treatment.

Original languageEnglish (US)
Pages (from-to)491-504
Number of pages14
JournalCancer Research
Volume76
Issue number2
DOIs
StatePublished - Jan 15 2016

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Adipocytes
Breast Neoplasms
Cytokines
Neoplasms
Neoplastic Stem Cells
Obesity
Mortality
Coculture Techniques
Cultured Cells
Up-Regulation
Stem Cells
Cell Culture Techniques
Fats
Morbidity

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Interactions between adipocytes and breast cancer cells stimulate cytokine production and drive Src/Sox2/miR-302b-mediated malignant progression. / Picon-Ruiz, Manuel; Pan, Chendong; Drews-Elger, Katherine; Jang, Kibeom; Besser, Alexandra H.; Zhao, Dekuang; Morata-Tarifa, Cynthia; Kim, Minsoon; Ince, Tan; Azzam, Diana J.; Wander, Seth A.; Wang, Bin; Ergonul, Burcu; Datar, Ram; Cote, Richard J; Howard, Guy; El-Ashry, Dorraya; Torné-Poyatos, Pablo; Marchal, Juan A.; Slingerland, Joyce M.

In: Cancer Research, Vol. 76, No. 2, 15.01.2016, p. 491-504.

Research output: Contribution to journalArticle

Picon-Ruiz, M, Pan, C, Drews-Elger, K, Jang, K, Besser, AH, Zhao, D, Morata-Tarifa, C, Kim, M, Ince, T, Azzam, DJ, Wander, SA, Wang, B, Ergonul, B, Datar, R, Cote, RJ, Howard, G, El-Ashry, D, Torné-Poyatos, P, Marchal, JA & Slingerland, JM 2016, 'Interactions between adipocytes and breast cancer cells stimulate cytokine production and drive Src/Sox2/miR-302b-mediated malignant progression', Cancer Research, vol. 76, no. 2, pp. 491-504. https://doi.org/10.1158/0008-5472.CAN-15-0927
Picon-Ruiz, Manuel ; Pan, Chendong ; Drews-Elger, Katherine ; Jang, Kibeom ; Besser, Alexandra H. ; Zhao, Dekuang ; Morata-Tarifa, Cynthia ; Kim, Minsoon ; Ince, Tan ; Azzam, Diana J. ; Wander, Seth A. ; Wang, Bin ; Ergonul, Burcu ; Datar, Ram ; Cote, Richard J ; Howard, Guy ; El-Ashry, Dorraya ; Torné-Poyatos, Pablo ; Marchal, Juan A. ; Slingerland, Joyce M. / Interactions between adipocytes and breast cancer cells stimulate cytokine production and drive Src/Sox2/miR-302b-mediated malignant progression. In: Cancer Research. 2016 ; Vol. 76, No. 2. pp. 491-504.
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abstract = "Consequences of the obesity epidemic on cancer morbidity and mortality are not fully appreciated. Obesity is a risk factor for many cancers, but the mechanisms by which it contributes to cancer development and patient outcome have yet to be fully elucidated. Here, we examined the effects of coculturing humanderived adipocytes with established and primary breast cancer cells on tumorigenic potential. We found that the interaction between adipocytes and cancer cells increased the secretion of proinflammatory cytokines. Prolonged culture of cancer cells with adipocytes or cytokines increased the proportion of mammosphereforming cells and of cells expressing stem-like markers in vitro. Furthermore, contact with immature adipocytes increased the abundance of cancer cells with tumor-forming and metastatic potential in vivo. Mechanistic investigations demonstrated that cancer cells cultured with immature adipocytes or cytokines activated Src, thus promoting Sox2, c-Myc, and Nanog upregulation. Moreover, Sox2-dependent induction of miR-302b further stimulated cMYC and SOX2 expression and potentiated the cytokineinduced cancer stem cell-like properties. Finally, we found that Src inhibitors decreased cytokine production after coculture, indicating that Src is not only activated by adipocyte or cytokine exposures, but is also required to sustain cytokine induction. These data support a model in which cancer cell invasion into local fat would establish feed-forward loops to activate Src, maintain proinflammatory cytokine production, and increase tumor-initiating cell abundance and metastatic progression. Collectively, our findings reveal new insights underlying increased breast cancer mortality in obese individuals and provide a novel preclinical rationale to test the efficacy of Src inhibitors for breast cancer treatment.",
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AU - Picon-Ruiz, Manuel

AU - Pan, Chendong

AU - Drews-Elger, Katherine

AU - Jang, Kibeom

AU - Besser, Alexandra H.

AU - Zhao, Dekuang

AU - Morata-Tarifa, Cynthia

AU - Kim, Minsoon

AU - Ince, Tan

AU - Azzam, Diana J.

AU - Wander, Seth A.

AU - Wang, Bin

AU - Ergonul, Burcu

AU - Datar, Ram

AU - Cote, Richard J

AU - Howard, Guy

AU - El-Ashry, Dorraya

AU - Torné-Poyatos, Pablo

AU - Marchal, Juan A.

AU - Slingerland, Joyce M

PY - 2016/1/15

Y1 - 2016/1/15

N2 - Consequences of the obesity epidemic on cancer morbidity and mortality are not fully appreciated. Obesity is a risk factor for many cancers, but the mechanisms by which it contributes to cancer development and patient outcome have yet to be fully elucidated. Here, we examined the effects of coculturing humanderived adipocytes with established and primary breast cancer cells on tumorigenic potential. We found that the interaction between adipocytes and cancer cells increased the secretion of proinflammatory cytokines. Prolonged culture of cancer cells with adipocytes or cytokines increased the proportion of mammosphereforming cells and of cells expressing stem-like markers in vitro. Furthermore, contact with immature adipocytes increased the abundance of cancer cells with tumor-forming and metastatic potential in vivo. Mechanistic investigations demonstrated that cancer cells cultured with immature adipocytes or cytokines activated Src, thus promoting Sox2, c-Myc, and Nanog upregulation. Moreover, Sox2-dependent induction of miR-302b further stimulated cMYC and SOX2 expression and potentiated the cytokineinduced cancer stem cell-like properties. Finally, we found that Src inhibitors decreased cytokine production after coculture, indicating that Src is not only activated by adipocyte or cytokine exposures, but is also required to sustain cytokine induction. These data support a model in which cancer cell invasion into local fat would establish feed-forward loops to activate Src, maintain proinflammatory cytokine production, and increase tumor-initiating cell abundance and metastatic progression. Collectively, our findings reveal new insights underlying increased breast cancer mortality in obese individuals and provide a novel preclinical rationale to test the efficacy of Src inhibitors for breast cancer treatment.

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