Interaction with the inositol 1,4,5-trisphosphate receptor promotes Ca2+ sequestration in permeabilised insulin-secreting cells

Md Shahidul Islam, Thomas Nilsson, Patrik Rorsman, Per Olof Berggren

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Electropermeabilised insulin-secreting RINm5F cells sequestered Ca2+, resulting in a steady-state level of the ambient free Ca2+ concentration corresponding to 723 ± 127 nM (mean ± SEM, n = 10), as monitored by a Ca2+-selective minielectrode. Inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) promoted a rapid and pronounced release of Ca2+. This Ca2+ was resequestered and a new steady-state Ca2+ level was attained, which was always lower (460 ± 102 nM, n = 10, P < 0.001) than the steady-state Ca2+ level maintained before the addition of Ins(1,4,5)P3. Whereas the initial reuptake of Ca2+ subsequent to Ins(1,4,5,)P3 stimulation was relatively slow, the later part of reuptake was fast as compared to the reuptake phases of a pulse addition of extraneous Ca2+. In the latter case the uptake of Ca2+ resulted in a steady-state level similar to that found in the absence of Ins(1,4,5)P3. Addition of Ins(1,4,5)P3 under this condition resulted in a further Ca2+ uptake and thus a lower steady-state Ca2+ level, Heparin, which binds to the Ins(1,4,5)P3 receptor, also lowered the steady-state free Ca2+ concentration, In contrast to Ins(1,4,5)P3 inositol 1,3,4,5-tetrakis-phosphate was without effect on Ca2+ sequestration. These findings are consistent with the presence of a high-affinity Ins(1,4,5)P3 receptor promoting continuous release of Ca2+ under basal conditions and/or the Ins(1,4,5)P3 receptor being actively involved in Ca2+ sequestration.

Original languageEnglish
Pages (from-to)27-29
Number of pages3
JournalFEBS Letters
Volume288
Issue number1-2
DOIs
StatePublished - Aug 19 1991
Externally publishedYes

Fingerprint

Inositol 1,4,5-Trisphosphate Receptors
Inositol 1,4,5-Trisphosphate
Insulin-Secreting Cells
Inositol
Heparin
Phosphates
Insulin
Scanning electron microscopy

Keywords

  • Inositol 1,4,5-trisphospate
  • Inositol 1,4,5-trisphosphate receptor
  • Insulin-secreting cell
  • Intracellular Ca-transport

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Interaction with the inositol 1,4,5-trisphosphate receptor promotes Ca2+ sequestration in permeabilised insulin-secreting cells. / Islam, Md Shahidul; Nilsson, Thomas; Rorsman, Patrik; Berggren, Per Olof.

In: FEBS Letters, Vol. 288, No. 1-2, 19.08.1991, p. 27-29.

Research output: Contribution to journalArticle

Islam, Md Shahidul ; Nilsson, Thomas ; Rorsman, Patrik ; Berggren, Per Olof. / Interaction with the inositol 1,4,5-trisphosphate receptor promotes Ca2+ sequestration in permeabilised insulin-secreting cells. In: FEBS Letters. 1991 ; Vol. 288, No. 1-2. pp. 27-29.
@article{d57f6d6a0d4f445aaa0a6b5780b03d01,
title = "Interaction with the inositol 1,4,5-trisphosphate receptor promotes Ca2+ sequestration in permeabilised insulin-secreting cells",
abstract = "Electropermeabilised insulin-secreting RINm5F cells sequestered Ca2+, resulting in a steady-state level of the ambient free Ca2+ concentration corresponding to 723 ± 127 nM (mean ± SEM, n = 10), as monitored by a Ca2+-selective minielectrode. Inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) promoted a rapid and pronounced release of Ca2+. This Ca2+ was resequestered and a new steady-state Ca2+ level was attained, which was always lower (460 ± 102 nM, n = 10, P < 0.001) than the steady-state Ca2+ level maintained before the addition of Ins(1,4,5)P3. Whereas the initial reuptake of Ca2+ subsequent to Ins(1,4,5,)P3 stimulation was relatively slow, the later part of reuptake was fast as compared to the reuptake phases of a pulse addition of extraneous Ca2+. In the latter case the uptake of Ca2+ resulted in a steady-state level similar to that found in the absence of Ins(1,4,5)P3. Addition of Ins(1,4,5)P3 under this condition resulted in a further Ca2+ uptake and thus a lower steady-state Ca2+ level, Heparin, which binds to the Ins(1,4,5)P3 receptor, also lowered the steady-state free Ca2+ concentration, In contrast to Ins(1,4,5)P3 inositol 1,3,4,5-tetrakis-phosphate was without effect on Ca2+ sequestration. These findings are consistent with the presence of a high-affinity Ins(1,4,5)P3 receptor promoting continuous release of Ca2+ under basal conditions and/or the Ins(1,4,5)P3 receptor being actively involved in Ca2+ sequestration.",
keywords = "Inositol 1,4,5-trisphospate, Inositol 1,4,5-trisphosphate receptor, Insulin-secreting cell, Intracellular Ca-transport",
author = "Islam, {Md Shahidul} and Thomas Nilsson and Patrik Rorsman and Berggren, {Per Olof}",
year = "1991",
month = "8",
day = "19",
doi = "10.1016/0014-5793(91)80995-F",
language = "English",
volume = "288",
pages = "27--29",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Interaction with the inositol 1,4,5-trisphosphate receptor promotes Ca2+ sequestration in permeabilised insulin-secreting cells

AU - Islam, Md Shahidul

AU - Nilsson, Thomas

AU - Rorsman, Patrik

AU - Berggren, Per Olof

PY - 1991/8/19

Y1 - 1991/8/19

N2 - Electropermeabilised insulin-secreting RINm5F cells sequestered Ca2+, resulting in a steady-state level of the ambient free Ca2+ concentration corresponding to 723 ± 127 nM (mean ± SEM, n = 10), as monitored by a Ca2+-selective minielectrode. Inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) promoted a rapid and pronounced release of Ca2+. This Ca2+ was resequestered and a new steady-state Ca2+ level was attained, which was always lower (460 ± 102 nM, n = 10, P < 0.001) than the steady-state Ca2+ level maintained before the addition of Ins(1,4,5)P3. Whereas the initial reuptake of Ca2+ subsequent to Ins(1,4,5,)P3 stimulation was relatively slow, the later part of reuptake was fast as compared to the reuptake phases of a pulse addition of extraneous Ca2+. In the latter case the uptake of Ca2+ resulted in a steady-state level similar to that found in the absence of Ins(1,4,5)P3. Addition of Ins(1,4,5)P3 under this condition resulted in a further Ca2+ uptake and thus a lower steady-state Ca2+ level, Heparin, which binds to the Ins(1,4,5)P3 receptor, also lowered the steady-state free Ca2+ concentration, In contrast to Ins(1,4,5)P3 inositol 1,3,4,5-tetrakis-phosphate was without effect on Ca2+ sequestration. These findings are consistent with the presence of a high-affinity Ins(1,4,5)P3 receptor promoting continuous release of Ca2+ under basal conditions and/or the Ins(1,4,5)P3 receptor being actively involved in Ca2+ sequestration.

AB - Electropermeabilised insulin-secreting RINm5F cells sequestered Ca2+, resulting in a steady-state level of the ambient free Ca2+ concentration corresponding to 723 ± 127 nM (mean ± SEM, n = 10), as monitored by a Ca2+-selective minielectrode. Inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) promoted a rapid and pronounced release of Ca2+. This Ca2+ was resequestered and a new steady-state Ca2+ level was attained, which was always lower (460 ± 102 nM, n = 10, P < 0.001) than the steady-state Ca2+ level maintained before the addition of Ins(1,4,5)P3. Whereas the initial reuptake of Ca2+ subsequent to Ins(1,4,5,)P3 stimulation was relatively slow, the later part of reuptake was fast as compared to the reuptake phases of a pulse addition of extraneous Ca2+. In the latter case the uptake of Ca2+ resulted in a steady-state level similar to that found in the absence of Ins(1,4,5)P3. Addition of Ins(1,4,5)P3 under this condition resulted in a further Ca2+ uptake and thus a lower steady-state Ca2+ level, Heparin, which binds to the Ins(1,4,5)P3 receptor, also lowered the steady-state free Ca2+ concentration, In contrast to Ins(1,4,5)P3 inositol 1,3,4,5-tetrakis-phosphate was without effect on Ca2+ sequestration. These findings are consistent with the presence of a high-affinity Ins(1,4,5)P3 receptor promoting continuous release of Ca2+ under basal conditions and/or the Ins(1,4,5)P3 receptor being actively involved in Ca2+ sequestration.

KW - Inositol 1,4,5-trisphospate

KW - Inositol 1,4,5-trisphosphate receptor

KW - Insulin-secreting cell

KW - Intracellular Ca-transport

UR - http://www.scopus.com/inward/record.url?scp=0025863609&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025863609&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(91)80995-F

DO - 10.1016/0014-5793(91)80995-F

M3 - Article

VL - 288

SP - 27

EP - 29

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1-2

ER -