Interaction of trophoblast membranes with lymphocytes and other cells.

J. A. McIntyre, W. P. Faulk, M. J. O'Sullivan

Research output: Contribution to journalArticle

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Abstract

Certain proteins present in trophoblast membranes may be instrumental in assuring the successful cohabitation of allogeneic tissues in the materno-fetal relationship of normal human pregnancy, inasmuch as preparations of trophoblast microvilli can specifically impede the progress of immune recognition reactions, as measured by the mixed lymphocyte culture (MLC) reaction. Microvilli prepared from fresh placentae were extracted with 3 mol/1 KCl and solubilized overnight in 1 per cent sodium deoxycholic acid (DOC). Following ultracentrifugation, the DOC supernatant chromatographed on Bio-Gel P-200 yielded two major protein peaks. The first peak (P1) was observed to eliminate MLC reactivity, impede the spontaneous turnover of T-cell-enriched populations and interfere with the proliferation of the Daudi lymphoblastoid B cell line. Sepharose CL-6B chromatography of KCl-extracted, DOC-solubilized microvilli yielded a broad peak (m.w. 5 X 10(4) to 5 X 10(5)); 5 micrograms of this specifically abrogated all MLC reactivity while leaving lymphocyte responses to lectins unaffected. The composition of the MLC-inhibitory peak was analysed by using SDS polyacrylamide gel electrophoresis under reducing conditions; it revealed 15 protein bands, six of which also stained for glycoproteins.

Original languageEnglish
Pages (from-to)95-102
Number of pages8
JournalPlacenta. Supplement
Volume3
StatePublished - Dec 1 1981

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Trophoblasts
Deoxycholic Acid
Microvilli
Lymphocytes
Membranes
Mixed Lymphocyte Culture Test
Proteins
Ultracentrifugation
Lectins
Placenta
Chromatography
Polyacrylamide Gel Electrophoresis
Glycoproteins
B-Lymphocytes
Gels
Sodium
T-Lymphocytes
Cell Line
Pregnancy
Population

ASJC Scopus subject areas

  • Medicine(all)

Cite this

McIntyre, J. A., Faulk, W. P., & O'Sullivan, M. J. (1981). Interaction of trophoblast membranes with lymphocytes and other cells. Placenta. Supplement, 3, 95-102.

Interaction of trophoblast membranes with lymphocytes and other cells. / McIntyre, J. A.; Faulk, W. P.; O'Sullivan, M. J.

In: Placenta. Supplement, Vol. 3, 01.12.1981, p. 95-102.

Research output: Contribution to journalArticle

McIntyre, JA, Faulk, WP & O'Sullivan, MJ 1981, 'Interaction of trophoblast membranes with lymphocytes and other cells.', Placenta. Supplement, vol. 3, pp. 95-102.
McIntyre JA, Faulk WP, O'Sullivan MJ. Interaction of trophoblast membranes with lymphocytes and other cells. Placenta. Supplement. 1981 Dec 1;3:95-102.
McIntyre, J. A. ; Faulk, W. P. ; O'Sullivan, M. J. / Interaction of trophoblast membranes with lymphocytes and other cells. In: Placenta. Supplement. 1981 ; Vol. 3. pp. 95-102.
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