Abstract
There is an increasing application of quantum dots (QDs) in plant science, as markers for the cells or their cell walls (CWs). In a plant cell the CW is a first target place for external agents. We studied interaction of CdSe QDs with CWs isolated from a conifer - Picea omorika (Panč) Purkyně branch. Binding of CdSe QDs was followed by using fluorescence microscopy, fluorescence and FT-IR spectroscopy. The aim of the study was to see whether the QDs induce structural changes in the CW, as well as to find out which kind of interactions between QDs and CWs occur and to which particular constituent polymers QDs preferably bind. The isolated CW is an appropriate object for study of the interactions with nanoparticles. The results show that in the CW, CdSe predominantly binds to cellulose, via OH groups and to lignin, via the conjugated C. C/C-C chains. The differences in interaction of wet and dry CWs with QDs/chloroform were also studied. In the reaction of the dry CW sample with QDs/chloroform, hydrophobic interactions are dominant. When water was added after QDs/chloroform, hydrophilic interactions enable a partial reconstruction of the C. C chains. The results have an implication on the use of the QDs in plant bio-imaging.
| Original language | English |
|---|---|
| Pages (from-to) | 41-47 |
| Number of pages | 7 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Volume | 91 |
| Issue number | 1 |
| DOIs | |
| State | Published - Mar 1 2012 |
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Keywords
- CdSe quantum dots
- Fluorescence spectroscopy
- Lignin
- Plant cell walls
- Spectral deconvolution
ASJC Scopus subject areas
- Biotechnology
- Colloid and Surface Chemistry
- Physical and Theoretical Chemistry
- Surfaces and Interfaces
Cite this
Interaction of the CdSe quantum dots with plant cell walls. / Djikanović, Daniela; Kalauzi, Aleksandar; Jeremić, Milorad; Xu, Jianmin; Mićić, Miodrag; Whyte, Jeffrey D.; Leblanc, Roger; Radotić, Ksenija.
In: Colloids and Surfaces B: Biointerfaces, Vol. 91, No. 1, 01.03.2012, p. 41-47.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Interaction of the CdSe quantum dots with plant cell walls
AU - Djikanović, Daniela
AU - Kalauzi, Aleksandar
AU - Jeremić, Milorad
AU - Xu, Jianmin
AU - Mićić, Miodrag
AU - Whyte, Jeffrey D.
AU - Leblanc, Roger
AU - Radotić, Ksenija
PY - 2012/3/1
Y1 - 2012/3/1
N2 - There is an increasing application of quantum dots (QDs) in plant science, as markers for the cells or their cell walls (CWs). In a plant cell the CW is a first target place for external agents. We studied interaction of CdSe QDs with CWs isolated from a conifer - Picea omorika (Panč) Purkyně branch. Binding of CdSe QDs was followed by using fluorescence microscopy, fluorescence and FT-IR spectroscopy. The aim of the study was to see whether the QDs induce structural changes in the CW, as well as to find out which kind of interactions between QDs and CWs occur and to which particular constituent polymers QDs preferably bind. The isolated CW is an appropriate object for study of the interactions with nanoparticles. The results show that in the CW, CdSe predominantly binds to cellulose, via OH groups and to lignin, via the conjugated C. C/C-C chains. The differences in interaction of wet and dry CWs with QDs/chloroform were also studied. In the reaction of the dry CW sample with QDs/chloroform, hydrophobic interactions are dominant. When water was added after QDs/chloroform, hydrophilic interactions enable a partial reconstruction of the C. C chains. The results have an implication on the use of the QDs in plant bio-imaging.
AB - There is an increasing application of quantum dots (QDs) in plant science, as markers for the cells or their cell walls (CWs). In a plant cell the CW is a first target place for external agents. We studied interaction of CdSe QDs with CWs isolated from a conifer - Picea omorika (Panč) Purkyně branch. Binding of CdSe QDs was followed by using fluorescence microscopy, fluorescence and FT-IR spectroscopy. The aim of the study was to see whether the QDs induce structural changes in the CW, as well as to find out which kind of interactions between QDs and CWs occur and to which particular constituent polymers QDs preferably bind. The isolated CW is an appropriate object for study of the interactions with nanoparticles. The results show that in the CW, CdSe predominantly binds to cellulose, via OH groups and to lignin, via the conjugated C. C/C-C chains. The differences in interaction of wet and dry CWs with QDs/chloroform were also studied. In the reaction of the dry CW sample with QDs/chloroform, hydrophobic interactions are dominant. When water was added after QDs/chloroform, hydrophilic interactions enable a partial reconstruction of the C. C chains. The results have an implication on the use of the QDs in plant bio-imaging.
KW - CdSe quantum dots
KW - Fluorescence spectroscopy
KW - Lignin
KW - Plant cell walls
KW - Spectral deconvolution
UR - http://www.scopus.com/inward/record.url?scp=84855204379&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84855204379&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2011.10.032
DO - 10.1016/j.colsurfb.2011.10.032
M3 - Article
C2 - 22104400
AN - SCOPUS:84855204379
VL - 91
SP - 41
EP - 47
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
IS - 1
ER -