TY - JOUR
T1 - Interaction of rhodamine 123 with mitochondria isolated from drug-sensitive and -resistant friend leukemia cells
AU - Abou-Khalil, Samir
AU - Abou-Khalil, Wafa H.
AU - Planas, Lilian
AU - Tapiero, Haim
AU - Lampidis, Theodore J.
N1 - Funding Information:
This work was supported in part by NIH Grant CA37109 to T.J.L.
PY - 1985/3/29
Y1 - 1985/3/29
N2 - Mitochondria isolated from Friend leukemia cell lines sensitive (FS) and resistant (FR) to rhodamine 123 (Rho123), showed respiratory control and ADP/O ratios indicative of well-coupled oxidative phosphorylation activity. When Rho123 was added to mitochondria from both cell lines, respiratory State 4 increased. The increase was higher in mitochondria isolated from resistant than from sensitive cells. Respiratory State 3 was slightly more inhibited by Rho123 in resistant than in sensitive cell mitochondria (98 and 82% inhibition, respectively). While it is not clear how the uncoupling-like effects of Rho123 on State 4 contribute to cellular toxicity, our results indicate that differential cellular sensitivity to the drug does not correlate with inhibition of oxidative phosphorylation in mitochondria isolated from drug-sensitive and resistant cells.
AB - Mitochondria isolated from Friend leukemia cell lines sensitive (FS) and resistant (FR) to rhodamine 123 (Rho123), showed respiratory control and ADP/O ratios indicative of well-coupled oxidative phosphorylation activity. When Rho123 was added to mitochondria from both cell lines, respiratory State 4 increased. The increase was higher in mitochondria isolated from resistant than from sensitive cells. Respiratory State 3 was slightly more inhibited by Rho123 in resistant than in sensitive cell mitochondria (98 and 82% inhibition, respectively). While it is not clear how the uncoupling-like effects of Rho123 on State 4 contribute to cellular toxicity, our results indicate that differential cellular sensitivity to the drug does not correlate with inhibition of oxidative phosphorylation in mitochondria isolated from drug-sensitive and resistant cells.
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U2 - 10.1016/S0006-291X(85)80049-8
DO - 10.1016/S0006-291X(85)80049-8
M3 - Article
C2 - 3985951
AN - SCOPUS:0021861241
VL - 127
SP - 1039
EP - 1044
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -