Interaction of rhodamine 123 with mitochondria isolated from drug-sensitive and -resistant friend leukemia cells

Samir Abou-Khalil, Wafa H. Abou-Khalil, Lilian Planas, Haim Tapiero, Theodore J. Lampidis

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Mitochondria isolated from Friend leukemia cell lines sensitive (FS) and resistant (FR) to rhodamine 123 (Rho123), showed respiratory control and ADP/O ratios indicative of well-coupled oxidative phosphorylation activity. When Rho123 was added to mitochondria from both cell lines, respiratory State 4 increased. The increase was higher in mitochondria isolated from resistant than from sensitive cells. Respiratory State 3 was slightly more inhibited by Rho123 in resistant than in sensitive cell mitochondria (98 and 82% inhibition, respectively). While it is not clear how the uncoupling-like effects of Rho123 on State 4 contribute to cellular toxicity, our results indicate that differential cellular sensitivity to the drug does not correlate with inhibition of oxidative phosphorylation in mitochondria isolated from drug-sensitive and resistant cells.

Original languageEnglish (US)
Pages (from-to)1039-1044
Number of pages6
JournalBiochemical and biophysical research communications
Volume127
Issue number3
DOIs
StatePublished - Mar 29 1985

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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