Interaction of rabbit lipoproteins and red blood cells with liposomes of egg yolk phospholipids

Armando J. Mendez, Jin Lin He, Hui Sheng Huang, Shu Rong Wen, S. L. Hsia

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

After intravenous injection of liposomes prepared from egg yolk phospholipids into rabbits, the phospholipids were readily assimilated by the lipoproteins, and there were increases in the circulating levels of cholesterol and phospholipids. The increases in cholesterol level were mainly due to increases of free cholesterol. Gradient ultracentrifugation showed that the lipoproteins decreased in density, and gel filtration chromatography showed that they increased in particle size. Upon electrophoresis, they exhibited slower mobility. Liposomes recovered from rabbits 3 hr after the injection contained free cholesterol, apolipoproteins A-I, E and traces of C. The apolipoprotein may target the liposomes for uptake by hepatocytes. Incubation of the liposomes with rabbit red blood cell membranes in vitro caused a decrease in cholesterol content of the membranes. However, the cholesterol/phosphate ratio in red blood cells isolated from the rabbits after the injection of liposomes did not change significantly, suggesting rapid replenishment of red blood cell cholesterol in vivo, possibly by equilibration with lipoprotein cholesterol or tissue cholesterol. These results suggest that the injection of phospholipid liposomes may have an antiatherogenic effect by the removal of tissue cholesterol and enhancing hepatic disposal of cholesterol through the reverse cholesterol transport mechanism.

Original languageEnglish (US)
Pages (from-to)961-967
Number of pages7
JournalLipids
Volume23
Issue number10
DOIs
StatePublished - Oct 1 1988

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

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