Integration is not necessary for expression of human immunodeficiency virus type 1 protein products

Mario Stevenson, Sheryl Haggerty, Carolyn A. Lamonica, Craig M. Meier, Siao Kun Welch, Andrzej J. Wasiak

Research output: Contribution to journalArticle

143 Citations (Scopus)

Abstract

A common feature in the life cycle of cytocidal retroviruses, including human immunodeficiency virus type 1 (HIV-1), is the accumulation of large amounts of unintegrated viral DNA. As yet, the role of unintegrated viral DNA in the cytopathogenesis of cytocidal retrovirus infections remains unresolved. HIV-1 mutants which were deleted in the integrase/endonuclease gene and which were unable to establish an integrated form of the virus were constructed. Despite an inability to integrate, these mutants were fully competent templates for HIV-1 core and envelope antigen production. HIV-1 antigen could be detected in the supernatants of lymphocyte cultures infected with HIV-1 integrase mutants. However, an inability to rescue infectious virus from these cultures indicated that HIV-1 integration was required for the production of infectious HIV-1. On the basis of the ability of unintegrated HIV-1 DNA to serve as a template for HIV-1 antigen production, it is plausible that unintegrated viral DNA can contribute to the HIV-1 antigen pool during HIV-1 replication.

Original languageEnglish
Pages (from-to)2421-2425
Number of pages5
JournalJournal of Virology
Volume64
Issue number5
StatePublished - Dec 1 1990
Externally publishedYes

Fingerprint

Human Immunodeficiency Virus Proteins
protein products
Human immunodeficiency virus 1
HIV-1
Viral DNA
Retroviridae
antigens
Antigens
DNA
mutants
Retroviridae Infections
Virus Integration
Viruses
Integrases
viruses
Virus Replication
Life Cycle Stages
life cycle (organisms)
lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Stevenson, M., Haggerty, S., Lamonica, C. A., Meier, C. M., Welch, S. K., & Wasiak, A. J. (1990). Integration is not necessary for expression of human immunodeficiency virus type 1 protein products. Journal of Virology, 64(5), 2421-2425.

Integration is not necessary for expression of human immunodeficiency virus type 1 protein products. / Stevenson, Mario; Haggerty, Sheryl; Lamonica, Carolyn A.; Meier, Craig M.; Welch, Siao Kun; Wasiak, Andrzej J.

In: Journal of Virology, Vol. 64, No. 5, 01.12.1990, p. 2421-2425.

Research output: Contribution to journalArticle

Stevenson, M, Haggerty, S, Lamonica, CA, Meier, CM, Welch, SK & Wasiak, AJ 1990, 'Integration is not necessary for expression of human immunodeficiency virus type 1 protein products', Journal of Virology, vol. 64, no. 5, pp. 2421-2425.
Stevenson M, Haggerty S, Lamonica CA, Meier CM, Welch SK, Wasiak AJ. Integration is not necessary for expression of human immunodeficiency virus type 1 protein products. Journal of Virology. 1990 Dec 1;64(5):2421-2425.
Stevenson, Mario ; Haggerty, Sheryl ; Lamonica, Carolyn A. ; Meier, Craig M. ; Welch, Siao Kun ; Wasiak, Andrzej J. / Integration is not necessary for expression of human immunodeficiency virus type 1 protein products. In: Journal of Virology. 1990 ; Vol. 64, No. 5. pp. 2421-2425.
@article{c5e889dfbf9c42fb8cf3d5e176743d7f,
title = "Integration is not necessary for expression of human immunodeficiency virus type 1 protein products",
abstract = "A common feature in the life cycle of cytocidal retroviruses, including human immunodeficiency virus type 1 (HIV-1), is the accumulation of large amounts of unintegrated viral DNA. As yet, the role of unintegrated viral DNA in the cytopathogenesis of cytocidal retrovirus infections remains unresolved. HIV-1 mutants which were deleted in the integrase/endonuclease gene and which were unable to establish an integrated form of the virus were constructed. Despite an inability to integrate, these mutants were fully competent templates for HIV-1 core and envelope antigen production. HIV-1 antigen could be detected in the supernatants of lymphocyte cultures infected with HIV-1 integrase mutants. However, an inability to rescue infectious virus from these cultures indicated that HIV-1 integration was required for the production of infectious HIV-1. On the basis of the ability of unintegrated HIV-1 DNA to serve as a template for HIV-1 antigen production, it is plausible that unintegrated viral DNA can contribute to the HIV-1 antigen pool during HIV-1 replication.",
author = "Mario Stevenson and Sheryl Haggerty and Lamonica, {Carolyn A.} and Meier, {Craig M.} and Welch, {Siao Kun} and Wasiak, {Andrzej J.}",
year = "1990",
month = "12",
day = "1",
language = "English",
volume = "64",
pages = "2421--2425",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "5",

}

TY - JOUR

T1 - Integration is not necessary for expression of human immunodeficiency virus type 1 protein products

AU - Stevenson, Mario

AU - Haggerty, Sheryl

AU - Lamonica, Carolyn A.

AU - Meier, Craig M.

AU - Welch, Siao Kun

AU - Wasiak, Andrzej J.

PY - 1990/12/1

Y1 - 1990/12/1

N2 - A common feature in the life cycle of cytocidal retroviruses, including human immunodeficiency virus type 1 (HIV-1), is the accumulation of large amounts of unintegrated viral DNA. As yet, the role of unintegrated viral DNA in the cytopathogenesis of cytocidal retrovirus infections remains unresolved. HIV-1 mutants which were deleted in the integrase/endonuclease gene and which were unable to establish an integrated form of the virus were constructed. Despite an inability to integrate, these mutants were fully competent templates for HIV-1 core and envelope antigen production. HIV-1 antigen could be detected in the supernatants of lymphocyte cultures infected with HIV-1 integrase mutants. However, an inability to rescue infectious virus from these cultures indicated that HIV-1 integration was required for the production of infectious HIV-1. On the basis of the ability of unintegrated HIV-1 DNA to serve as a template for HIV-1 antigen production, it is plausible that unintegrated viral DNA can contribute to the HIV-1 antigen pool during HIV-1 replication.

AB - A common feature in the life cycle of cytocidal retroviruses, including human immunodeficiency virus type 1 (HIV-1), is the accumulation of large amounts of unintegrated viral DNA. As yet, the role of unintegrated viral DNA in the cytopathogenesis of cytocidal retrovirus infections remains unresolved. HIV-1 mutants which were deleted in the integrase/endonuclease gene and which were unable to establish an integrated form of the virus were constructed. Despite an inability to integrate, these mutants were fully competent templates for HIV-1 core and envelope antigen production. HIV-1 antigen could be detected in the supernatants of lymphocyte cultures infected with HIV-1 integrase mutants. However, an inability to rescue infectious virus from these cultures indicated that HIV-1 integration was required for the production of infectious HIV-1. On the basis of the ability of unintegrated HIV-1 DNA to serve as a template for HIV-1 antigen production, it is plausible that unintegrated viral DNA can contribute to the HIV-1 antigen pool during HIV-1 replication.

UR - http://www.scopus.com/inward/record.url?scp=0025350783&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025350783&partnerID=8YFLogxK

M3 - Article

C2 - 2157898

AN - SCOPUS:0025350783

VL - 64

SP - 2421

EP - 2425

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 5

ER -