Integration is not necessary for expression of human immunodeficiency virus type 1 protein products

M. Stevenson, S. Haggerty, C. A. Lamonica, C. M. Meier, S. K. Welch, A. J. Wasiak

Research output: Contribution to journalArticlepeer-review

149 Scopus citations

Abstract

A common feature in the life cycle of cytocidal retroviruses, including human immunodeficiency virus type 1 (HIV-1), is the accumulation of large amounts of unintegrated viral DNA. As yet, the role of unintegrated viral DNA in the cytopathogenesis of cytocidal retrovirus infections remains unresolved. HIV-1 mutants which were deleted in the integrase/endonuclease gene and which were unable to establish an integrated form of the virus were constructed. Despite an inability to integrate, these mutants were fully competent templates for HIV-1 core and envelope antigen production. HIV-1 antigen could be detected in the supernatants of lymphocyte cultures infected with HIV-1 integrase mutants. However, an inability to rescue infectious virus from these cultures indicated that HIV-1 integration was required for the production of infectious HIV-1. On the basis of the ability of unintegrated HIV-1 DNA to serve as a template for HIV-1 antigen production, it is plausible that unintegrated viral DNA can contribute to the HIV-1 antigen pool during HIV-1 replication.

Original languageEnglish (US)
Pages (from-to)2421-2425
Number of pages5
JournalJournal of virology
Volume64
Issue number5
DOIs
StatePublished - 1990
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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