Integrated platform for operating and interrogating organs-on-chips

Matthew Ishahak, Liev Birman, Daniel Carbonero, Jordan Hill, Adiel Hernandez, Siddarth Rawal, Ashutosh Agarwal

Research output: Contribution to journalArticle

Abstract

The development of microphysiological systems, also known as organs-on-chips, has highlighted the need for improved in vitro models for drug discovery. However, the highly specialized skillset required to design, build, and operate these systems has hindered adoption. Here, we describe an integrated platform that enables both continuous perifusion culture and dynamic cell secretion assays. We also developed a graphical user interface that allows the user to not only control the hardware components, but also define automated programs. The system's functionality was validated by maintaining pancreatic islets in culture under continuous perifusion for 24 hours, then performing a functional glucose stimulated insulin secretion assay. We demonstrate that islets remained both viable and functional on the platform with minimal user involvement.

Original languageEnglish (US)
Pages (from-to)5645-5651
Number of pages7
JournalAnalytical Methods
Volume11
Issue number43
DOIs
StatePublished - Nov 21 2019

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Assays
Insulin
Graphical user interfaces
Glucose
Hardware
Drug Discovery

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Engineering(all)

Cite this

Ishahak, M., Birman, L., Carbonero, D., Hill, J., Hernandez, A., Rawal, S., & Agarwal, A. (2019). Integrated platform for operating and interrogating organs-on-chips. Analytical Methods, 11(43), 5645-5651. https://doi.org/10.1039/c9ay01663e

Integrated platform for operating and interrogating organs-on-chips. / Ishahak, Matthew; Birman, Liev; Carbonero, Daniel; Hill, Jordan; Hernandez, Adiel; Rawal, Siddarth; Agarwal, Ashutosh.

In: Analytical Methods, Vol. 11, No. 43, 21.11.2019, p. 5645-5651.

Research output: Contribution to journalArticle

Ishahak, M, Birman, L, Carbonero, D, Hill, J, Hernandez, A, Rawal, S & Agarwal, A 2019, 'Integrated platform for operating and interrogating organs-on-chips', Analytical Methods, vol. 11, no. 43, pp. 5645-5651. https://doi.org/10.1039/c9ay01663e
Ishahak M, Birman L, Carbonero D, Hill J, Hernandez A, Rawal S et al. Integrated platform for operating and interrogating organs-on-chips. Analytical Methods. 2019 Nov 21;11(43):5645-5651. https://doi.org/10.1039/c9ay01663e
Ishahak, Matthew ; Birman, Liev ; Carbonero, Daniel ; Hill, Jordan ; Hernandez, Adiel ; Rawal, Siddarth ; Agarwal, Ashutosh. / Integrated platform for operating and interrogating organs-on-chips. In: Analytical Methods. 2019 ; Vol. 11, No. 43. pp. 5645-5651.
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