Insulin-like growth factor II mRNA expression in human breast cancer

D. Yee, K. J. Cullen, S. Paik, J. F. Perdue, B. Hampton, A. Schwartz, Marc E Lippman, N. Rosen

Research output: Contribution to journalArticle

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Abstract

Insulin-like growth factor II is a growth factor important in fetal development. Several cancer tissues and cell lines have been reported to express IGF-II and rat IGF-II is mitogenic for breast cancer cell lines. Using Northern analysis and ribonuclease protection assays, IGF-II mRNA was detected in normal fibroblasts and in the established breast cancer cell line, T47D. In this cell line, steady state levels of IGF-II message were increased by treatment with estradiol. 10 nM IGF-II, purified from human serum, was mitogenic for breast cancer cell lines. In vitro, IGF-II may act as an autocrine growth factor for some cell lines. RNA derived from breast cancer, pathologically normal breast tissue, and benign breast disease also contained IGF-II mRNA. When paired samples of normal and cancer tissue were obtained from the breast of the same patient, the level of IGF-II mRNA expression in the normal tissue was at least that found in the cancer. This is consistent with previous observations that show IGF-II is expressed in mesenchyme. These findings suggest that in breast cancer IGF-II is produced by stromal tissue elements and potentially by the malignant epithelial cells. Therefore, IGF-II may function as an autocrine or a paracrine growth factor in different breast tumors.

Original languageEnglish
Pages (from-to)6691-6696
Number of pages6
JournalCancer Research
Volume48
Issue number23
StatePublished - Dec 1 1988
Externally publishedYes

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Insulin-Like Growth Factor II
Breast Neoplasms
Messenger RNA
Cell Line
Intercellular Signaling Peptides and Proteins
Breast
Neoplasms
Breast Diseases
Mesoderm
Ribonucleases
Fetal Development
Estradiol
Fibroblasts
Epithelial Cells
RNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Yee, D., Cullen, K. J., Paik, S., Perdue, J. F., Hampton, B., Schwartz, A., ... Rosen, N. (1988). Insulin-like growth factor II mRNA expression in human breast cancer. Cancer Research, 48(23), 6691-6696.

Insulin-like growth factor II mRNA expression in human breast cancer. / Yee, D.; Cullen, K. J.; Paik, S.; Perdue, J. F.; Hampton, B.; Schwartz, A.; Lippman, Marc E; Rosen, N.

In: Cancer Research, Vol. 48, No. 23, 01.12.1988, p. 6691-6696.

Research output: Contribution to journalArticle

Yee, D, Cullen, KJ, Paik, S, Perdue, JF, Hampton, B, Schwartz, A, Lippman, ME & Rosen, N 1988, 'Insulin-like growth factor II mRNA expression in human breast cancer', Cancer Research, vol. 48, no. 23, pp. 6691-6696.
Yee D, Cullen KJ, Paik S, Perdue JF, Hampton B, Schwartz A et al. Insulin-like growth factor II mRNA expression in human breast cancer. Cancer Research. 1988 Dec 1;48(23):6691-6696.
Yee, D. ; Cullen, K. J. ; Paik, S. ; Perdue, J. F. ; Hampton, B. ; Schwartz, A. ; Lippman, Marc E ; Rosen, N. / Insulin-like growth factor II mRNA expression in human breast cancer. In: Cancer Research. 1988 ; Vol. 48, No. 23. pp. 6691-6696.
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