Insulin-like growth factor II inhibits glucose-induced insulin exocytosis

Qimin Zhang, Per Olof Berggren, Olof Larsson, Kerstin Hall, Michael Tally

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

We have investigated the effect of IGF-II on glucose-induced insulin release in the pancreatic β-cell. Introduction of IGF-II during perifusion of the cells with 20 mM glucose abolished glucose-induced insulin release. Concomitant addition of IGF-II with 20 mM glucose caused a complete inhibition of insulin release. In addition, IGF-II inhibited Ca2+-induced insulin release from electropermeabilized pancreatic β-cells. IGF-II had no effect on K+- or tolbutamide-induced insulin release. However, IGF-II could suppress K+-stimulated insulin release when cells were pretreated with the protein phosphatase inhibitor okadaic acid. The inhibitory effect of IGF-II on insulin release was not associated with significant changes in membrane potential, activity of the voltage gated L-type Ca2+-channel or cytoplasmic free Ca2+ concentration. Pretreatment of the cells with pertussis toxin or the phorbol ester TPA abolished the inhibitory action of IGF-II on insulin release. Hence, the molecular mechanism whereby activation of the IGF-II/M6P receptor by IGF-II inhibits glucose-stimulated insulin exocytosis in the pancreatic β-cell involves pertussis toxin-sensitive G proteins and is dependent on PKC activity.

Original languageEnglish (US)
Pages (from-to)117-121
Number of pages5
JournalBiochemical and biophysical research communications
Volume243
Issue number1
DOIs
StatePublished - Feb 4 1998

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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