Abstract
Calcium uptake by skinned muscle fibers is stimulated by physiological concentrations of insulin. These fibers, which lack a functional plasma membrane, are permeable to macromolecules but retain extensive portions of their sarcolemma in the form of transverse tubules intercalated between the myofibrils. They have an active sarcoplasmic reticulum that removes 45Ca2+ from solution at concentrations below the threshold that initiates contraction (< 1μM). The Ca2+ uptake activity is stimulated by insulin, presumably in response to its binding to those receptors located in the transverse tubules. Addition of glucose 6-phosphate, whose intracellular concentration increases in response to insulin, also stimulates Ca2+ uptake, a unique property of this preparation. These data indicate that insulin and glucose 6-phosphate act in concert to stimulate the sarcoplasmic reticulum. The resulting decrease in myoplasmic Ca2+ and the increase in glucose 6-phosphate would serve to mediate some of the anabolic effects of the hormone.
| Original language | English |
|---|---|
| Pages (from-to) | 936-939 |
| Number of pages | 4 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 77 |
| Issue number | 2 II |
| State | Published - Jan 1 1980 |
| Externally published | Yes |
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ASJC Scopus subject areas
- General
- Genetics
Cite this
Insulin and glucose-6-phosphate stimulation of Ca2+ uptake by skinned muscle fibers. / Brautigan, D. L.; Kerrick, W. Glenn; Fischer, E. H.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 77, No. 2 II, 01.01.1980, p. 936-939.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Insulin and glucose-6-phosphate stimulation of Ca2+ uptake by skinned muscle fibers
AU - Brautigan, D. L.
AU - Kerrick, W. Glenn
AU - Fischer, E. H.
PY - 1980/1/1
Y1 - 1980/1/1
N2 - Calcium uptake by skinned muscle fibers is stimulated by physiological concentrations of insulin. These fibers, which lack a functional plasma membrane, are permeable to macromolecules but retain extensive portions of their sarcolemma in the form of transverse tubules intercalated between the myofibrils. They have an active sarcoplasmic reticulum that removes 45Ca2+ from solution at concentrations below the threshold that initiates contraction (< 1μM). The Ca2+ uptake activity is stimulated by insulin, presumably in response to its binding to those receptors located in the transverse tubules. Addition of glucose 6-phosphate, whose intracellular concentration increases in response to insulin, also stimulates Ca2+ uptake, a unique property of this preparation. These data indicate that insulin and glucose 6-phosphate act in concert to stimulate the sarcoplasmic reticulum. The resulting decrease in myoplasmic Ca2+ and the increase in glucose 6-phosphate would serve to mediate some of the anabolic effects of the hormone.
AB - Calcium uptake by skinned muscle fibers is stimulated by physiological concentrations of insulin. These fibers, which lack a functional plasma membrane, are permeable to macromolecules but retain extensive portions of their sarcolemma in the form of transverse tubules intercalated between the myofibrils. They have an active sarcoplasmic reticulum that removes 45Ca2+ from solution at concentrations below the threshold that initiates contraction (< 1μM). The Ca2+ uptake activity is stimulated by insulin, presumably in response to its binding to those receptors located in the transverse tubules. Addition of glucose 6-phosphate, whose intracellular concentration increases in response to insulin, also stimulates Ca2+ uptake, a unique property of this preparation. These data indicate that insulin and glucose 6-phosphate act in concert to stimulate the sarcoplasmic reticulum. The resulting decrease in myoplasmic Ca2+ and the increase in glucose 6-phosphate would serve to mediate some of the anabolic effects of the hormone.
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M3 - Article
C2 - 6987674
AN - SCOPUS:0018817476
VL - 77
SP - 936
EP - 939
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 2 II
ER -