Insulin and glucose-6-phosphate stimulation of Ca2+ uptake by skinned muscle fibers

D. L. Brautigan, W. Glenn Kerrick, E. H. Fischer

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Calcium uptake by skinned muscle fibers is stimulated by physiological concentrations of insulin. These fibers, which lack a functional plasma membrane, are permeable to macromolecules but retain extensive portions of their sarcolemma in the form of transverse tubules intercalated between the myofibrils. They have an active sarcoplasmic reticulum that removes 45Ca2+ from solution at concentrations below the threshold that initiates contraction (< 1μM). The Ca2+ uptake activity is stimulated by insulin, presumably in response to its binding to those receptors located in the transverse tubules. Addition of glucose 6-phosphate, whose intracellular concentration increases in response to insulin, also stimulates Ca2+ uptake, a unique property of this preparation. These data indicate that insulin and glucose 6-phosphate act in concert to stimulate the sarcoplasmic reticulum. The resulting decrease in myoplasmic Ca2+ and the increase in glucose 6-phosphate would serve to mediate some of the anabolic effects of the hormone.

Original languageEnglish
Pages (from-to)936-939
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume77
Issue number2 II
StatePublished - Jan 1 1980
Externally publishedYes

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Glucose-6-Phosphate
Insulin
Muscles
Sarcoplasmic Reticulum
Anabolic Agents
Sarcolemma
Myofibrils
Cell Membrane
Hormones
Calcium

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Insulin and glucose-6-phosphate stimulation of Ca2+ uptake by skinned muscle fibers. / Brautigan, D. L.; Kerrick, W. Glenn; Fischer, E. H.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 77, No. 2 II, 01.01.1980, p. 936-939.

Research output: Contribution to journalArticle

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