Instability of IS6110 patterns in multidrug-resistant strains of Mycobacterium tuberculosis

P. Farnia, M. R. Masjedi, B. Nasiri, Mehdi Mirsaeidi, S. Sorooch, M. Kazeampour, A. A. Velayati

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The stability of IS6110 restriction fragment length polymorphism (RFLP) pattern was determined in 31 isolates from patients with multidrug-resistant tuberculosis (MDR-TB). These patients were in actual chains of transmission and they referred to the National Institute of Tuberculosis and Lung Diseases, Tehran, Iran. Susceptibility testing against first- and second-line drugs were performed by the proportional method on Lowenstein-Jensen culture media. Thereafter, DNA fingerprinting by IS6110 with direct repeat (DR) region as a probe was performed by standard protocols. The rate of IS6110 changes was 16%, although, no variation was found in the DR region, in a time-span of 1-63 months. The strains with unstable IS6110 patterns were resistant to all drugs tested, and the majority of them (60%) were collected from HIV-positive patients. The results demonstrated that for a reliable interpretation of strain typing, it is better to use an additional marker along with IS6110 RFLP.

Original languageEnglish (US)
Pages (from-to)346-352
Number of pages7
JournalEpidemiology and Infection
Volume135
Issue number2
DOIs
StatePublished - Feb 2007
Externally publishedYes

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Mycobacterium tuberculosis
Nucleic Acid Repetitive Sequences
Restriction Fragment Length Polymorphisms
Multidrug-Resistant Tuberculosis
DNA Fingerprinting
Iran
Pharmaceutical Preparations
Lung Diseases
Culture Media
Tuberculosis
HIV

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Farnia, P., Masjedi, M. R., Nasiri, B., Mirsaeidi, M., Sorooch, S., Kazeampour, M., & Velayati, A. A. (2007). Instability of IS6110 patterns in multidrug-resistant strains of Mycobacterium tuberculosis. Epidemiology and Infection, 135(2), 346-352. https://doi.org/10.1017/S0950268806006790

Instability of IS6110 patterns in multidrug-resistant strains of Mycobacterium tuberculosis. / Farnia, P.; Masjedi, M. R.; Nasiri, B.; Mirsaeidi, Mehdi; Sorooch, S.; Kazeampour, M.; Velayati, A. A.

In: Epidemiology and Infection, Vol. 135, No. 2, 02.2007, p. 346-352.

Research output: Contribution to journalArticle

Farnia, P, Masjedi, MR, Nasiri, B, Mirsaeidi, M, Sorooch, S, Kazeampour, M & Velayati, AA 2007, 'Instability of IS6110 patterns in multidrug-resistant strains of Mycobacterium tuberculosis', Epidemiology and Infection, vol. 135, no. 2, pp. 346-352. https://doi.org/10.1017/S0950268806006790
Farnia, P. ; Masjedi, M. R. ; Nasiri, B. ; Mirsaeidi, Mehdi ; Sorooch, S. ; Kazeampour, M. ; Velayati, A. A. / Instability of IS6110 patterns in multidrug-resistant strains of Mycobacterium tuberculosis. In: Epidemiology and Infection. 2007 ; Vol. 135, No. 2. pp. 346-352.
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