Instability of immunoglobulin genes in S107 cell line

Seung-Uon Shin, Ronald DePinho, Donald J. Zack, Stuart Rudikoff, Matthew D. Scharff

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Somatic mutation occurs frequently in rearranged and expressed immunoglobulin variable region genes in vivo. In contrast, V region hypermutation seldom occurs in antibody-forming cells in culture. The S107 mouse myeloma cell line is one of the few cell lines that has been observed to generate V region mutations frequently and spontaneously in vitro. Detailed examination reveals that both the S107 tumor and the cell line derived from it contain and express a duplicated heavy-chain gene. In culture, only one of the two heavy-chain genes undergoes both V and C region mutation, and variants with complex phenotypes and genotypes arise as a result of mutation and segregation of these duplicated genes.

Original languageEnglish
Pages (from-to)259-276
Number of pages18
JournalSomatic Cell and Molecular Genetics
Volume17
Issue number3
DOIs
StatePublished - May 1 1991
Externally publishedYes

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Immunoglobulin Genes
Cell Line
Mutation
Genes
Immunoglobulin Variable Region
Tumor Cell Line
Cell Culture Techniques
Genotype
Phenotype
Antibodies

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Instability of immunoglobulin genes in S107 cell line. / Shin, Seung-Uon; DePinho, Ronald; Zack, Donald J.; Rudikoff, Stuart; Scharff, Matthew D.

In: Somatic Cell and Molecular Genetics, Vol. 17, No. 3, 01.05.1991, p. 259-276.

Research output: Contribution to journalArticle

Shin, S-U, DePinho, R, Zack, DJ, Rudikoff, S & Scharff, MD 1991, 'Instability of immunoglobulin genes in S107 cell line', Somatic Cell and Molecular Genetics, vol. 17, no. 3, pp. 259-276. https://doi.org/10.1007/BF01232821
Shin, Seung-Uon ; DePinho, Ronald ; Zack, Donald J. ; Rudikoff, Stuart ; Scharff, Matthew D. / Instability of immunoglobulin genes in S107 cell line. In: Somatic Cell and Molecular Genetics. 1991 ; Vol. 17, No. 3. pp. 259-276.
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