Abstract
The mRNA levels of a set of immune-related genes were analysed with peripheral blood samples from at-risk, new-onset and long-term type 1 diabetes (T1D) patients, in comparison to those from healthy controls. The selected set includes T lymphocyte genes [CD3G and cytotoxic T lymphocyte-associated antigen 4 (CTLA4)], B lymphocyte genes (CD19 and CD20) and myeloid cell-related genes [CD11b, Toll-like receptor (TLR)-9, arginase (ARG1)]. Also included is a subset of the S100 family members that has been documented recently as regulatory elements of innate immunity. Samples from patients with long-term T1D had a reduced level of mRNA for most of selected innate and adaptive immune genes. No such reduction was detected in samples collected from at-risk or new-onset T1D patients. Analyses of regulatory gene expression ratios revealed a dynamic disproportion of CTLA4 versus CD3G expression in samples from at-risk, new-onset and long-term T1D patients. These changes could serve as immunological biomarkers for the status of the immune system during T1D progression and therapeutic interventions.
| Original language | English |
|---|---|
| Pages (from-to) | 131-138 |
| Number of pages | 8 |
| Journal | Clinical and Experimental Immunology |
| Volume | 170 |
| Issue number | 2 |
| DOIs | |
| State | Published - Nov 1 2012 |
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Keywords
- Autoimmunity
- CTLA4
- Diabetes
- Gene expression
- Human
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
Cite this
Innate and adaptive immune gene expression profiles as biomarkers in human type 1 diabetes. / Han, D.; Cai, Xiaodong; Wen, J.; Matheson, D.; Skyler, Jay S; Kenyon, Norma S; Chen, Zhibin.
In: Clinical and Experimental Immunology, Vol. 170, No. 2, 01.11.2012, p. 131-138.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Innate and adaptive immune gene expression profiles as biomarkers in human type 1 diabetes
AU - Han, D.
AU - Cai, Xiaodong
AU - Wen, J.
AU - Matheson, D.
AU - Skyler, Jay S
AU - Kenyon, Norma S
AU - Chen, Zhibin
PY - 2012/11/1
Y1 - 2012/11/1
N2 - The mRNA levels of a set of immune-related genes were analysed with peripheral blood samples from at-risk, new-onset and long-term type 1 diabetes (T1D) patients, in comparison to those from healthy controls. The selected set includes T lymphocyte genes [CD3G and cytotoxic T lymphocyte-associated antigen 4 (CTLA4)], B lymphocyte genes (CD19 and CD20) and myeloid cell-related genes [CD11b, Toll-like receptor (TLR)-9, arginase (ARG1)]. Also included is a subset of the S100 family members that has been documented recently as regulatory elements of innate immunity. Samples from patients with long-term T1D had a reduced level of mRNA for most of selected innate and adaptive immune genes. No such reduction was detected in samples collected from at-risk or new-onset T1D patients. Analyses of regulatory gene expression ratios revealed a dynamic disproportion of CTLA4 versus CD3G expression in samples from at-risk, new-onset and long-term T1D patients. These changes could serve as immunological biomarkers for the status of the immune system during T1D progression and therapeutic interventions.
AB - The mRNA levels of a set of immune-related genes were analysed with peripheral blood samples from at-risk, new-onset and long-term type 1 diabetes (T1D) patients, in comparison to those from healthy controls. The selected set includes T lymphocyte genes [CD3G and cytotoxic T lymphocyte-associated antigen 4 (CTLA4)], B lymphocyte genes (CD19 and CD20) and myeloid cell-related genes [CD11b, Toll-like receptor (TLR)-9, arginase (ARG1)]. Also included is a subset of the S100 family members that has been documented recently as regulatory elements of innate immunity. Samples from patients with long-term T1D had a reduced level of mRNA for most of selected innate and adaptive immune genes. No such reduction was detected in samples collected from at-risk or new-onset T1D patients. Analyses of regulatory gene expression ratios revealed a dynamic disproportion of CTLA4 versus CD3G expression in samples from at-risk, new-onset and long-term T1D patients. These changes could serve as immunological biomarkers for the status of the immune system during T1D progression and therapeutic interventions.
KW - Autoimmunity
KW - CTLA4
KW - Diabetes
KW - Gene expression
KW - Human
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UR - http://www.scopus.com/inward/citedby.url?scp=84867221309&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2249.2012.04650.x
DO - 10.1111/j.1365-2249.2012.04650.x
M3 - Article
C2 - 23039883
AN - SCOPUS:84867221309
VL - 170
SP - 131
EP - 138
JO - Clinical and Experimental Immunology
JF - Clinical and Experimental Immunology
SN - 0009-9104
IS - 2
ER -