Injectable form of cross-linked hyaluronan is effective for middle ear wound healing

Simon Angeli, Sarah Connell, Brian Gibson, Ali Ozdek, John T. McElveen, Thomas R. Van De Water

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Objectives: Two studies were designed to investigate a hyaluronan (HA) gel for middle ear (ME) wound healing. Methods: We used a guinea pig model of ME wound healing. In a long-term study, we performed a comparison of hearing and ME inflammation in 3 groups. Group 1 (n = 8) underwent bilateral wounding of ME mucosa and unilateral packing of the ME with HA gel (Sepragel). Group 2 (n = 6) was the same as group 1 except that the packing was absorbable bovine collagen sponges (Gelfoam). Group 3, the control group (n = 14), had operated, unpacked ears. In a short-term study, we investigated ME retention of HA gel at 1 and 2 weeks (n = 16). Results: At 1 week, all ears showed decreased distortion product otoacoustic emissions (DPOAEs) and auditory brain stem responses (ABRs) secondary to ME packing and postsurgical inflammation. The controls recovered preoperative DPOAEs and ABRs by week 2. Group 1 had decreased low-frequency DPOAEs at weeks 2 and 6, but their high-frequency DPOAEs and ABRs recovered to preoperative values by week 6. Group 2 had hearing losses that persisted throughout the study. Group 1 showed normal ME and inner ear histologic characteristics. Group 2 showed inflammatory cells within the ME and cochleas. Group 1 showed less packing retention than did group 2 at week 6 (p = .016). Eighty-five percent of the HA packing remained at 1 week, and 73% at 2 weeks. Conclusions: Hyaluronan gel was a relatively safe and effective ME packing material in our animal model.

Original languageEnglish (US)
Pages (from-to)667-673
Number of pages7
JournalAnnals of Otology, Rhinology and Laryngology
Issue number9 I
StatePublished - Sep 2007


  • Adhesion
  • Hyaluronan
  • Hyaluronic acid
  • Middle ear
  • Ototoxicity
  • Tympanoplasty

ASJC Scopus subject areas

  • Otorhinolaryngology


Dive into the research topics of 'Injectable form of cross-linked hyaluronan is effective for middle ear wound healing'. Together they form a unique fingerprint.

Cite this