Injectable alginate seeded with chondrocytes as a potential treatment for vesicoureteral reflux

A. Atala, L. G. Cima, W. Kim, K. T. Paige, J. P. Vacanti, A. B. Retik, C. A. Vacanti

Research output: Contribution to journalArticle

181 Citations (Scopus)

Abstract

Injection of polytetrafluoroethylene (Teflon) or collagen has been used in the endoscopic treatment of vesicoureteral reflux. Although the principle of an endoscopic treatment is valid, there are concerns regarding the long-term safety and effectiveness of these substances. The goal of several investigators has been to find alternate implant materials that would be safe for human use. Toward this goal we conducted a study to determine the effect of chondrocytes using a biodegradable polymer solution as a template. Hyaline cartilage was obtained from the articular surfaces of calf shoulders and chondrocytes were harvested. Chondrocyte suspensions were concentrated to 20, 30 and 40 x 106 cells per cc and mixed with dry alginate powder (a biodegradable polymer) to form a gel. Twelve athymic mice were injected subcutaneously with a chondrocyte-alginate solution. Each mouse had 4 injection sites, consisting of control, 10, 15 and 20 x 106 chondrocyte cells (48 injection sites). Mice were sacrificed at 2, 4, 6 and 12 weeks after injection. Histological examination of the injection sites demonstrated evidence of cartilage formation in 34 of the 36 experimental injection sites. Gross examination of the injection sites with increasing time showed that the polymer gels were progressively replaced by cartilage. The ultimate size of the cartilage formed was related to the initial chondrocyte concentration injected, and appeared to be uniform and stable within each category. There was no evidence of cartilage formation in the 12 controls. Histological analyses of distant organs showed no evidence of cartilage or alginate gel migration, or granuloma formation. In conclusion, chondrocyte-alginate gel suspensions are injectable, appear to be nonmigratory and are able to conserve their volume. In addition, the use of autologous cartilage cells would preclude an immunological reaction. These preliminary studies indicate that autologous cartilage-polymer gel solutions may be potentially useful in the endoscopic treatment of reflux.

Original languageEnglish
Pages (from-to)745-747
Number of pages3
JournalJournal of Urology
Volume150
Issue number2 SUPPL.
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Vesico-Ureteral Reflux
Chondrocytes
Cartilage
Injections
Gels
Polymers
Polytetrafluoroethylene
Therapeutics
Suspensions
Hyaline Cartilage
alginic acid
Granuloma
Nude Mice
Powders
Collagen
Joints
Research Personnel
Safety

Keywords

  • cartilage
  • endoscopy
  • vesico-ureteral reflux

ASJC Scopus subject areas

  • Urology

Cite this

Atala, A., Cima, L. G., Kim, W., Paige, K. T., Vacanti, J. P., Retik, A. B., & Vacanti, C. A. (1993). Injectable alginate seeded with chondrocytes as a potential treatment for vesicoureteral reflux. Journal of Urology, 150(2 SUPPL.), 745-747.

Injectable alginate seeded with chondrocytes as a potential treatment for vesicoureteral reflux. / Atala, A.; Cima, L. G.; Kim, W.; Paige, K. T.; Vacanti, J. P.; Retik, A. B.; Vacanti, C. A.

In: Journal of Urology, Vol. 150, No. 2 SUPPL., 01.01.1993, p. 745-747.

Research output: Contribution to journalArticle

Atala, A, Cima, LG, Kim, W, Paige, KT, Vacanti, JP, Retik, AB & Vacanti, CA 1993, 'Injectable alginate seeded with chondrocytes as a potential treatment for vesicoureteral reflux', Journal of Urology, vol. 150, no. 2 SUPPL., pp. 745-747.
Atala A, Cima LG, Kim W, Paige KT, Vacanti JP, Retik AB et al. Injectable alginate seeded with chondrocytes as a potential treatment for vesicoureteral reflux. Journal of Urology. 1993 Jan 1;150(2 SUPPL.):745-747.
Atala, A. ; Cima, L. G. ; Kim, W. ; Paige, K. T. ; Vacanti, J. P. ; Retik, A. B. ; Vacanti, C. A. / Injectable alginate seeded with chondrocytes as a potential treatment for vesicoureteral reflux. In: Journal of Urology. 1993 ; Vol. 150, No. 2 SUPPL. pp. 745-747.
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