Initiation of RNA Synthesis in Isolated Nuclei

Eli GILBOA, Hermona SOREQ, Haim AVIV

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Ribonucleotide triphosphates, labeled at the β position, were synthesized and used directly to quantify RNA chain initiation in nuclei isolated from Friend cells grown in tissue culture. At the optimal salt concentration, low‐molecular‐weight RNAs (4–5S) synthesized by RNA polymerase III were the predominant species initiated. Less than 5% of the molecules were initiated by polymerase II. We calculate that 50–80% of the small RNA molecules synthesized in vitro were also initiated in vitro. Assuming that a substantial fraction of the nuclei were active in vitro, the number of 4–5S RNA molecules initiated per nucleus was about 100 molecules/min.

Original languageEnglish (US)
Pages (from-to)393-400
Number of pages8
JournalEuropean Journal of Biochemistry
Volume77
Issue number2
DOIs
StatePublished - Jul 1977
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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