Initiation of ribosome degradation during starvation in Escherichia coli

Michael A. Zundel, Georgeta N. Basturea, Murray P Deutscher

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Ribosomes are known to be degraded under conditions of nutrient limitation. However, the mechanism by which a normally stable ribosome becomes a substrate for the degradation machinery has remained elusive. Here, we present in vitro and in vivo data demonstrating that free ribosome subunits are the actual targets of the degradative enzymes, whereas 70S particles are protected from such degradation. Conditions that increase the formation of subunits both in vitro and in vivo lead0 to enhanced degradation, while conditions favoring the presence of intact 70S ribosomes prevent or reduce breakdown. Thus, the simple formation of free 50S and 30S subunits is sufficient to serve as the initiation mechanism that allows endoribonuclease cleavage and subsequent ribosome breakdown.

Original languageEnglish
Pages (from-to)977-983
Number of pages7
JournalRNA
Volume15
Issue number5
DOIs
StatePublished - May 1 2009

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Starvation
Ribosomes
Escherichia coli
Endoribonucleases
Ribosome Subunits
Food
Enzymes
In Vitro Techniques

Keywords

  • Endoribonuclease
  • R
  • Ribosome subunits

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Zundel, M. A., Basturea, G. N., & Deutscher, M. P. (2009). Initiation of ribosome degradation during starvation in Escherichia coli. RNA, 15(5), 977-983. https://doi.org/10.1261/rna.1381309

Initiation of ribosome degradation during starvation in Escherichia coli. / Zundel, Michael A.; Basturea, Georgeta N.; Deutscher, Murray P.

In: RNA, Vol. 15, No. 5, 01.05.2009, p. 977-983.

Research output: Contribution to journalArticle

Zundel, MA, Basturea, GN & Deutscher, MP 2009, 'Initiation of ribosome degradation during starvation in Escherichia coli', RNA, vol. 15, no. 5, pp. 977-983. https://doi.org/10.1261/rna.1381309
Zundel, Michael A. ; Basturea, Georgeta N. ; Deutscher, Murray P. / Initiation of ribosome degradation during starvation in Escherichia coli. In: RNA. 2009 ; Vol. 15, No. 5. pp. 977-983.
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