Initial isolation and analysis of the human Kv1.7 (KCNA7) gene, a member of the voltage-gated potassium channel gene family

Vladimir I. Kashuba, Sergei M. Kvasha, Alexei I. Protopopov, Rinat Z. Gizatullin, Alla V. Rynditch, Claes R Wahlestedt, Wyeth W. Wasserman, Eugene R. Zabarovsky

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A novel human potassium channel gene was identified and isolated. The maximal open reading frame encodes a protein of 456 amino acids. The predicted product exhibits 91% amino acid identity to the murine voltage-gated potassium channel protein Kv1.7 (Kcna7), which plays an important role in the repolarization of cell membranes. Based on the high similarity, the human gene has been classified as the ortholog of the mouse Kcna7 and given the name Kv1.7 (KCNA7). A structural prediction identified a pore region characteristic of potassium channels and six membrane-spanning domains. Northern expression analysis revealed the gene is expressed preferentially in skeletal muscle, heart and kidney. However, it is expressed at lower level in other tissues, including liver. A single mRNA isoform was observed, with a size of approximately 4.5 kb. Using fluorescence in situ hybridization, the gene was mapped to chromosomal band 19q13.4 (269.13 cR3000). A genomic sequence was identified in the database from this region, and the KCNA7 gene structure determined. Computational analysis of the genomic sequence reveals the location of a putative promoter and a likely muscle-specific regulatory region. Initial comparison to the published murine Kcna7 cDNA suggested a different N-terminal sequence for the human protein, however, further analysis suggests that the original mouse sequence contained an error or an unusual polymorphism.

Original languageEnglish
Pages (from-to)115-122
Number of pages8
JournalGene
Volume268
Issue number1-2
DOIs
StatePublished - May 2 2001
Externally publishedYes

Fingerprint

Voltage-Gated Potassium Channels
Genes
Potassium Channels
RNA Isoforms
Amino Acids
Proteins
Nucleic Acid Regulatory Sequences
Fluorescence In Situ Hybridization
Open Reading Frames
Names
Sequence Analysis
Skeletal Muscle
Complementary DNA
Cell Membrane
Databases
Kidney
Muscles
Membranes
Liver

Keywords

  • Gene mapping
  • Gene structure
  • NotI-linking clone

ASJC Scopus subject areas

  • Genetics

Cite this

Kashuba, V. I., Kvasha, S. M., Protopopov, A. I., Gizatullin, R. Z., Rynditch, A. V., Wahlestedt, C. R., ... Zabarovsky, E. R. (2001). Initial isolation and analysis of the human Kv1.7 (KCNA7) gene, a member of the voltage-gated potassium channel gene family. Gene, 268(1-2), 115-122. https://doi.org/10.1016/S0378-1119(01)00423-1

Initial isolation and analysis of the human Kv1.7 (KCNA7) gene, a member of the voltage-gated potassium channel gene family. / Kashuba, Vladimir I.; Kvasha, Sergei M.; Protopopov, Alexei I.; Gizatullin, Rinat Z.; Rynditch, Alla V.; Wahlestedt, Claes R; Wasserman, Wyeth W.; Zabarovsky, Eugene R.

In: Gene, Vol. 268, No. 1-2, 02.05.2001, p. 115-122.

Research output: Contribution to journalArticle

Kashuba, VI, Kvasha, SM, Protopopov, AI, Gizatullin, RZ, Rynditch, AV, Wahlestedt, CR, Wasserman, WW & Zabarovsky, ER 2001, 'Initial isolation and analysis of the human Kv1.7 (KCNA7) gene, a member of the voltage-gated potassium channel gene family', Gene, vol. 268, no. 1-2, pp. 115-122. https://doi.org/10.1016/S0378-1119(01)00423-1
Kashuba, Vladimir I. ; Kvasha, Sergei M. ; Protopopov, Alexei I. ; Gizatullin, Rinat Z. ; Rynditch, Alla V. ; Wahlestedt, Claes R ; Wasserman, Wyeth W. ; Zabarovsky, Eugene R. / Initial isolation and analysis of the human Kv1.7 (KCNA7) gene, a member of the voltage-gated potassium channel gene family. In: Gene. 2001 ; Vol. 268, No. 1-2. pp. 115-122.
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