Inhibitory effect of calcitonin gene-related peptide on myometrial contractility is diminished at parturition

M. Naghashpour, M. I. Rosenblatt, I. M. Dickerson, G. P. Dahl

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

The uterus is innervated by calcitonin gene-related peptide (CORP) immunoreactive neurons, and CGRP inhibits spontaneous and evoked contractions in the uterus and fallopian tubes. In the present study using isometric force measurements on myometrial strips, we determined that CORP inhibition of acetylcholine-induced contractions was drastically reduced at parturition compared with earlier stages of pregnancy in mice. The levels of inhibition exerted by CORP paralleled the expression of a novel protein recently implicated in CGRP receptor activation, the CORP-receptor component protein (CGRP-RCP). The mouse CORP-RCP complementary DNA was isolated from uterus, and expression of the CGRP-RCP was monitored during gestation by Northern and Western blot analysis. Although CGRP-RCP messenger RNA levels did not vary significantly during gestation and postpartum, CGRP-RCP protein was greatly diminished at parturition. This diminution correlated with the loss of CGRP inhibition of acetylcholine-induced contractions observed in the force experiments. A role for CGRP and CGRP-RCP in modulation of myometrial smooth muscle contractility during pregnancy and in labor is suggested.

Original languageEnglish (US)
Pages (from-to)4207-4214
Number of pages8
JournalEndocrinology
Volume138
Issue number10
DOIs
StatePublished - 1997

ASJC Scopus subject areas

  • Endocrinology

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