Inhibition of vascular endothelial cells by 1,4-phenylenebis (methylene)selenocyanate - A novel chemopreventive organoselenium compound

J. J. Schumacher, P. Upadhyaya, Sundaram Ramakrishnan

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Organoselenium compound 1,4-phenylenebis(methylene)selenocyanate (p-XSC) was investigated for its effects on endothelial cell proliferation in vitro and angiogenesis in vivo. The organoselenium compound, p-XSC, has been shown to prevent carcinogen- induced tumorigenesis in murine model systems with low toxicity. Since tumor growth and metastasis are dependent on angiogenesis, we investigated the effects of the organoselenium compound on this process. Human umbilical vein endothelial cells treated with p-XSC showed concentration dependent inhibition of protein synthesis and cell viability in vitro with a TCID50 value of 6 μM. Subsequently, we studied the effects of p-XSC on experimental angiogenesis. Addition of p-XSC to three-dimensional cultures inhibited endothelial cell tube formation. Furthermore, p-XSC treatment inhibited growth factor induced angiogenesis in chick chorioallantoic membrane assays and i.p. administration of p-XSC inhibited neovascularization induced by tumor cells implanted subcutaneously into athymic mice. These studies suggest that vascular endothelium is an additional target for the chemopreventive organo-selenium compound p-XSC.

Original languageEnglish (US)
Pages (from-to)1945-1951
Number of pages7
JournalAnticancer Research
Volume21
Issue number3 B
StatePublished - Aug 20 2001
Externally publishedYes

Fingerprint

Organoselenium Compounds
Endothelial Cells
Selenium Compounds
Chorioallantoic Membrane
Angiogenesis Inducing Agents
Human Umbilical Vein Endothelial Cells
Vascular Endothelium
Nude Mice
Carcinogens
Neoplasms
Cell Survival
Intercellular Signaling Peptides and Proteins
Carcinogenesis
Cell Proliferation
Neoplasm Metastasis
Growth
1,4-phenylenebis(methylene)selenocyanate
Proteins
In Vitro Techniques

Keywords

  • Angiogenesis
  • Chemotherapy
  • Organoselenium
  • Tumor vasculature

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Inhibition of vascular endothelial cells by 1,4-phenylenebis (methylene)selenocyanate - A novel chemopreventive organoselenium compound. / Schumacher, J. J.; Upadhyaya, P.; Ramakrishnan, Sundaram.

In: Anticancer Research, Vol. 21, No. 3 B, 20.08.2001, p. 1945-1951.

Research output: Contribution to journalArticle

@article{0b40885dfe2f4dcd8dde7a734aaa3ef0,
title = "Inhibition of vascular endothelial cells by 1,4-phenylenebis (methylene)selenocyanate - A novel chemopreventive organoselenium compound",
abstract = "Organoselenium compound 1,4-phenylenebis(methylene)selenocyanate (p-XSC) was investigated for its effects on endothelial cell proliferation in vitro and angiogenesis in vivo. The organoselenium compound, p-XSC, has been shown to prevent carcinogen- induced tumorigenesis in murine model systems with low toxicity. Since tumor growth and metastasis are dependent on angiogenesis, we investigated the effects of the organoselenium compound on this process. Human umbilical vein endothelial cells treated with p-XSC showed concentration dependent inhibition of protein synthesis and cell viability in vitro with a TCID50 value of 6 μM. Subsequently, we studied the effects of p-XSC on experimental angiogenesis. Addition of p-XSC to three-dimensional cultures inhibited endothelial cell tube formation. Furthermore, p-XSC treatment inhibited growth factor induced angiogenesis in chick chorioallantoic membrane assays and i.p. administration of p-XSC inhibited neovascularization induced by tumor cells implanted subcutaneously into athymic mice. These studies suggest that vascular endothelium is an additional target for the chemopreventive organo-selenium compound p-XSC.",
keywords = "Angiogenesis, Chemotherapy, Organoselenium, Tumor vasculature",
author = "Schumacher, {J. J.} and P. Upadhyaya and Sundaram Ramakrishnan",
year = "2001",
month = "8",
day = "20",
language = "English (US)",
volume = "21",
pages = "1945--1951",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "3 B",

}

TY - JOUR

T1 - Inhibition of vascular endothelial cells by 1,4-phenylenebis (methylene)selenocyanate - A novel chemopreventive organoselenium compound

AU - Schumacher, J. J.

AU - Upadhyaya, P.

AU - Ramakrishnan, Sundaram

PY - 2001/8/20

Y1 - 2001/8/20

N2 - Organoselenium compound 1,4-phenylenebis(methylene)selenocyanate (p-XSC) was investigated for its effects on endothelial cell proliferation in vitro and angiogenesis in vivo. The organoselenium compound, p-XSC, has been shown to prevent carcinogen- induced tumorigenesis in murine model systems with low toxicity. Since tumor growth and metastasis are dependent on angiogenesis, we investigated the effects of the organoselenium compound on this process. Human umbilical vein endothelial cells treated with p-XSC showed concentration dependent inhibition of protein synthesis and cell viability in vitro with a TCID50 value of 6 μM. Subsequently, we studied the effects of p-XSC on experimental angiogenesis. Addition of p-XSC to three-dimensional cultures inhibited endothelial cell tube formation. Furthermore, p-XSC treatment inhibited growth factor induced angiogenesis in chick chorioallantoic membrane assays and i.p. administration of p-XSC inhibited neovascularization induced by tumor cells implanted subcutaneously into athymic mice. These studies suggest that vascular endothelium is an additional target for the chemopreventive organo-selenium compound p-XSC.

AB - Organoselenium compound 1,4-phenylenebis(methylene)selenocyanate (p-XSC) was investigated for its effects on endothelial cell proliferation in vitro and angiogenesis in vivo. The organoselenium compound, p-XSC, has been shown to prevent carcinogen- induced tumorigenesis in murine model systems with low toxicity. Since tumor growth and metastasis are dependent on angiogenesis, we investigated the effects of the organoselenium compound on this process. Human umbilical vein endothelial cells treated with p-XSC showed concentration dependent inhibition of protein synthesis and cell viability in vitro with a TCID50 value of 6 μM. Subsequently, we studied the effects of p-XSC on experimental angiogenesis. Addition of p-XSC to three-dimensional cultures inhibited endothelial cell tube formation. Furthermore, p-XSC treatment inhibited growth factor induced angiogenesis in chick chorioallantoic membrane assays and i.p. administration of p-XSC inhibited neovascularization induced by tumor cells implanted subcutaneously into athymic mice. These studies suggest that vascular endothelium is an additional target for the chemopreventive organo-selenium compound p-XSC.

KW - Angiogenesis

KW - Chemotherapy

KW - Organoselenium

KW - Tumor vasculature

UR - http://www.scopus.com/inward/record.url?scp=0034908538&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034908538&partnerID=8YFLogxK

M3 - Article

C2 - 11497282

AN - SCOPUS:0034908538

VL - 21

SP - 1945

EP - 1951

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 3 B

ER -