Abstract
We have studied scrovalentinoside, an iridoid with anti-inflammatory properties isolated from Scrophularia auriculata ssp. pseudoauriculata, as an anti-inflammatory agent in different experimental models of delayed-type hypersensitivity. We found that scrovalentinoside reduced the edema induced by oxazolone at 0.5 mg/ear and sheep red blood cells at 10 mg/kg. The observed effect occurred during the last phase or inflammatory response; during the earlier phase or induction of the delayed-type hypersensitivity reaction, no significant activity was noted. Thus, scrovalentinoside reduced both the edema and cell infiltration in vivo and reduced lymphocyte proliferation in vitro, affecting the cycle principally during the first 48 h. Whereas cells stimulated with phytohemagglutinin changed from the G0/G1 phase to the S and G2/M phases, when these same cells were treated with scrovalentinoside (100 μM), they remained in the G0/G1 phase. Finally, scrovalentinoside inhibited the production of the pro-inflammatory mediators' TNF-α, IFN-γ, IL-1β, IL-2, IL-4, LTB4, and NO, but had no effect on the production of the anti-inflammatory cytokine IL-10.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 419-427 |
| Number of pages | 9 |
| Journal | Journal of Ethnopharmacology |
| Volume | 110 |
| Issue number | 3 |
| DOIs | |
| State | Published - Apr 4 2007 |
| Externally published | Yes |
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Keywords
- Anti-inflammatory activity
- Cytokines
- Iridoids
- Leukotriene B
- Nitric oxide
- Nitric oxide synthase
- Scrophularia auriculata
- Scrovalentinoside
ASJC Scopus subject areas
- Pharmacology
Cite this
Inhibition of the pro-inflammatory mediators' production and anti-inflammatory effect of the iridoid scrovalentinoside. / Bas Infante, Esperanza; Recio, M. Carmen; Abdallah, Mohamed; Máñez, Salvador; Giner, Rosa M.; Cerdá-Nicolás, Miguel; Ríos, José Luis.
In: Journal of Ethnopharmacology, Vol. 110, No. 3, 04.04.2007, p. 419-427.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inhibition of the pro-inflammatory mediators' production and anti-inflammatory effect of the iridoid scrovalentinoside
AU - Bas Infante, Esperanza
AU - Recio, M. Carmen
AU - Abdallah, Mohamed
AU - Máñez, Salvador
AU - Giner, Rosa M.
AU - Cerdá-Nicolás, Miguel
AU - Ríos, José Luis
PY - 2007/4/4
Y1 - 2007/4/4
N2 - We have studied scrovalentinoside, an iridoid with anti-inflammatory properties isolated from Scrophularia auriculata ssp. pseudoauriculata, as an anti-inflammatory agent in different experimental models of delayed-type hypersensitivity. We found that scrovalentinoside reduced the edema induced by oxazolone at 0.5 mg/ear and sheep red blood cells at 10 mg/kg. The observed effect occurred during the last phase or inflammatory response; during the earlier phase or induction of the delayed-type hypersensitivity reaction, no significant activity was noted. Thus, scrovalentinoside reduced both the edema and cell infiltration in vivo and reduced lymphocyte proliferation in vitro, affecting the cycle principally during the first 48 h. Whereas cells stimulated with phytohemagglutinin changed from the G0/G1 phase to the S and G2/M phases, when these same cells were treated with scrovalentinoside (100 μM), they remained in the G0/G1 phase. Finally, scrovalentinoside inhibited the production of the pro-inflammatory mediators' TNF-α, IFN-γ, IL-1β, IL-2, IL-4, LTB4, and NO, but had no effect on the production of the anti-inflammatory cytokine IL-10.
AB - We have studied scrovalentinoside, an iridoid with anti-inflammatory properties isolated from Scrophularia auriculata ssp. pseudoauriculata, as an anti-inflammatory agent in different experimental models of delayed-type hypersensitivity. We found that scrovalentinoside reduced the edema induced by oxazolone at 0.5 mg/ear and sheep red blood cells at 10 mg/kg. The observed effect occurred during the last phase or inflammatory response; during the earlier phase or induction of the delayed-type hypersensitivity reaction, no significant activity was noted. Thus, scrovalentinoside reduced both the edema and cell infiltration in vivo and reduced lymphocyte proliferation in vitro, affecting the cycle principally during the first 48 h. Whereas cells stimulated with phytohemagglutinin changed from the G0/G1 phase to the S and G2/M phases, when these same cells were treated with scrovalentinoside (100 μM), they remained in the G0/G1 phase. Finally, scrovalentinoside inhibited the production of the pro-inflammatory mediators' TNF-α, IFN-γ, IL-1β, IL-2, IL-4, LTB4, and NO, but had no effect on the production of the anti-inflammatory cytokine IL-10.
KW - Anti-inflammatory activity
KW - Cytokines
KW - Iridoids
KW - Leukotriene B
KW - Nitric oxide
KW - Nitric oxide synthase
KW - Scrophularia auriculata
KW - Scrovalentinoside
UR - http://www.scopus.com/inward/record.url?scp=33847728203&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33847728203&partnerID=8YFLogxK
U2 - 10.1016/j.jep.2006.09.038
DO - 10.1016/j.jep.2006.09.038
M3 - Article
VL - 110
SP - 419
EP - 427
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
SN - 0378-8741
IS - 3
ER -