TY - JOUR
T1 - Inhibition of [3H]dopamine and [3H]serotonin uptake by cocaine
T2 - Comparison between chopped tissue slices and synaptosomes
AU - Izenwasser, Sari
AU - Rosenberger, John G.
AU - Cox, Brian M.
N1 - Funding Information:
We thank Dr. Peter Munson for providing the program AIIfit. This work was supported by a grant from the National Institute on Drug Abuse. The opinions and assertions contained herein are the private ones of the authors and are not to be construed as reflecting the views of the Department of Defense or the Uniformed Services University of the Health Sciences. Animals used for this study were acquired and cared for in accordance with the guidelines published in the NIH Guide for the Care and Use of Laboratory Animals (National Institutes of Health Publication No. 85-23), Revised 1985.
PY - 1992
Y1 - 1992
N2 - Cocaine inhibits both [3H]dopamine and [3H]serotonin uptake in rat striatum and nucleus accumbens. In a chopped tissue slice preparation, the inhibition curve for [3H]dopamine uptake is biphasic, suggesting two components of uptake, whereas the curve for [3H]serotonin uptake is steep and apparently monophasic. In synaptosomal preparations, both curves are monophasic. Monensin, a sodium ionophore, inhibits uptake but does not change the shape of the cocaine inhibition curve in synaptosomes, suggesting that the biphasic inhibition curves in slices are not likely due to differential sodium gradients across the slices. In tissue slices, only the component which is more sensitive to inhibition by cocaine and related drugs is inhibitable by nicotine. This suggests that the two components of dopamine uptake in tissue slices may be differentially regulated.
AB - Cocaine inhibits both [3H]dopamine and [3H]serotonin uptake in rat striatum and nucleus accumbens. In a chopped tissue slice preparation, the inhibition curve for [3H]dopamine uptake is biphasic, suggesting two components of uptake, whereas the curve for [3H]serotonin uptake is steep and apparently monophasic. In synaptosomal preparations, both curves are monophasic. Monensin, a sodium ionophore, inhibits uptake but does not change the shape of the cocaine inhibition curve in synaptosomes, suggesting that the biphasic inhibition curves in slices are not likely due to differential sodium gradients across the slices. In tissue slices, only the component which is more sensitive to inhibition by cocaine and related drugs is inhibitable by nicotine. This suggests that the two components of dopamine uptake in tissue slices may be differentially regulated.
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U2 - 10.1016/0024-3205(92)90365-V
DO - 10.1016/0024-3205(92)90365-V
M3 - Article
C2 - 1736024
AN - SCOPUS:0026610062
VL - 50
SP - 541
EP - 547
JO - Life Sciences
JF - Life Sciences
SN - 0024-3205
IS - 8
ER -