Abstract
Cocaine inhibits both [3H]dopamine and [3H]serotonin uptake in rat striatum and nucleus accumbens. In a chopped tissue slice preparation, the inhibition curve for [3H]dopamine uptake is biphasic, suggesting two components of uptake, whereas the curve for [3H]serotonin uptake is steep and apparently monophasic. In synaptosomal preparations, both curves are monophasic. Monensin, a sodium ionophore, inhibits uptake but does not change the shape of the cocaine inhibition curve in synaptosomes, suggesting that the biphasic inhibition curves in slices are not likely due to differential sodium gradients across the slices. In tissue slices, only the component which is more sensitive to inhibition by cocaine and related drugs is inhibitable by nicotine. This suggests that the two components of dopamine uptake in tissue slices may be differentially regulated.
| Original language | English |
|---|---|
| Pages (from-to) | 541-547 |
| Number of pages | 7 |
| Journal | Life Sciences |
| Volume | 50 |
| Issue number | 8 |
| DOIs | |
| State | Published - Jan 1 1992 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Pharmacology
Cite this
Inhibition of [3H]dopamine and [3H]serotonin uptake by cocaine : Comparison between chopped tissue slices and synaptosomes. / Izenwasser, Sari E; Rosenberger, John G.; Cox, Brian M.
In: Life Sciences, Vol. 50, No. 8, 01.01.1992, p. 541-547.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inhibition of [3H]dopamine and [3H]serotonin uptake by cocaine
T2 - Comparison between chopped tissue slices and synaptosomes
AU - Izenwasser, Sari E
AU - Rosenberger, John G.
AU - Cox, Brian M.
PY - 1992/1/1
Y1 - 1992/1/1
N2 - Cocaine inhibits both [3H]dopamine and [3H]serotonin uptake in rat striatum and nucleus accumbens. In a chopped tissue slice preparation, the inhibition curve for [3H]dopamine uptake is biphasic, suggesting two components of uptake, whereas the curve for [3H]serotonin uptake is steep and apparently monophasic. In synaptosomal preparations, both curves are monophasic. Monensin, a sodium ionophore, inhibits uptake but does not change the shape of the cocaine inhibition curve in synaptosomes, suggesting that the biphasic inhibition curves in slices are not likely due to differential sodium gradients across the slices. In tissue slices, only the component which is more sensitive to inhibition by cocaine and related drugs is inhibitable by nicotine. This suggests that the two components of dopamine uptake in tissue slices may be differentially regulated.
AB - Cocaine inhibits both [3H]dopamine and [3H]serotonin uptake in rat striatum and nucleus accumbens. In a chopped tissue slice preparation, the inhibition curve for [3H]dopamine uptake is biphasic, suggesting two components of uptake, whereas the curve for [3H]serotonin uptake is steep and apparently monophasic. In synaptosomal preparations, both curves are monophasic. Monensin, a sodium ionophore, inhibits uptake but does not change the shape of the cocaine inhibition curve in synaptosomes, suggesting that the biphasic inhibition curves in slices are not likely due to differential sodium gradients across the slices. In tissue slices, only the component which is more sensitive to inhibition by cocaine and related drugs is inhibitable by nicotine. This suggests that the two components of dopamine uptake in tissue slices may be differentially regulated.
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UR - http://www.scopus.com/inward/citedby.url?scp=0026610062&partnerID=8YFLogxK
U2 - 10.1016/0024-3205(92)90365-V
DO - 10.1016/0024-3205(92)90365-V
M3 - Article
C2 - 1736024
AN - SCOPUS:0026610062
VL - 50
SP - 541
EP - 547
JO - Life Sciences
JF - Life Sciences
SN - 0024-3205
IS - 8
ER -